Flavocytochrome b2-Based Enzymatic Method of L-Lactate Assay in Food Products
Joint Authors
Karkovska, Maria
Smutok, Halyna
Gonchar, Mykhailo V.
Smutok, Oleh V.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-24
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
L-lactate, a key metabolite of the anaerobic glycolytic pathway, plays an important role as a biomarker in medicine, in the nutritional sector and food quality control.
For these reasons, there is a need for very specific, sensitive, and simple analytical methods for the accurate L-lactate measuring.
A new highly selective enzymatic method for L-lactate determination based on the use of flavocytochrome b2 (EC 1.1.2.3; FC b2) isolated from the recombinant strain of the yeast Hansenula polymorpha has been developed.
A proposed enzymatic method exploits an enzymatic oxidation of L-lactate to pyruvate coupled with nitrotetrazolium blue (NTZB) reduction to a colored product, formazan.
The maximal absorption peak of the colored product is near λ=525 nm and the linear range is observed in the interval 0.005–0.14 mM of L-lactate.
The main advantages of the proposed method when compared to the LDH-based routine approaches are a higher sensitivity (2.0 μM of L-lactate), simple procedure of analysis, usage of inexpensive, nontoxic reagents, and small amount of the enzyme.
Enzymatic oxidation of L-lactate catalyzed by flavocytochrome b2 and coupled with formazan production from nitrotetrazolium blue was shown to be used for L-lactate assay in food samples.
A high correlation between results of the proposed method and reference ones proves the possibility to use flavocytochrome b2-catalysed reaction for enzymatic measurement of L-lactate in biotechnology and food chemistry.
American Psychological Association (APA)
Smutok, Oleh V.& Karkovska, Maria& Smutok, Halyna& Gonchar, Mykhailo V.. 2013. Flavocytochrome b2-Based Enzymatic Method of L-Lactate Assay in Food Products. The Scientific World Journal،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1032944
Modern Language Association (MLA)
Smutok, Oleh V.…[et al.]. Flavocytochrome b2-Based Enzymatic Method of L-Lactate Assay in Food Products. The Scientific World Journal No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-1032944
American Medical Association (AMA)
Smutok, Oleh V.& Karkovska, Maria& Smutok, Halyna& Gonchar, Mykhailo V.. Flavocytochrome b2-Based Enzymatic Method of L-Lactate Assay in Food Products. The Scientific World Journal. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-1032944
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1032944