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Involvement of MAPKs and PLC Pathways in Modulation of Pacemaking Activity by So-Cheong-Ryong-Tang in Interstitial Cells of Cajal from Murine Small Intestine
Joint Authors
Hwang, Minwoo
Lee, Hee Jung
Song, Ho Joon
Kim, Byung Joo
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-10-30
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Purpose.
Interstitial cells of Cajal (ICCs) are the pacemaker cells that generate slow waves in the gastrointestinal (GI) tract.
We have aimed to investigate the effects of Socheongryong-Tang (SCRT) in ICCs from mouse’s small intestine.
Methods.
The whole-cell patch-clamp configuration was used to record membrane potentials from cultured ICCs.
Intracellular Ca2+ ([Ca2+]i) increase was studied in cultured ICCs using fura-2 AM.
Results.
ICCs generated pacemaker potentials in mouse’s small intestine.
SCRT produced membrane depolarization in current clamp mode.
Y25130 (5-HT3 receptor antagonist) and RS39604 (5-HT4 receptor antagonist) blocked SCRT-induced membrane depolarizations, whereas SB269970 (5-HT7 receptor antagonist) did not.
When GDP-β-S (1 mM) was in the pipette solution, SCRT did not induce the membrane depolarizations.
[Ca2+]i analysis showed that SCRT increased [Ca2+]i.
In the presence of PD98059 (p42/44 MAPK inhibitor), SCRT did not produce membrane depolarizations.
In addition, SB203580 (p38 MAPK inhibitor) and JNK inhibitors blocked the depolarizations by SCRT in pacemaker potentials.
Furthermore, the membrane depolarizations by SCRT were not inhibited by U-73122, an active phospholipase C (PLC) inhibitor, but by U-73343, an inactive PLC inhibitor.
Conclusion.
These results suggest that SCRT might affect GI motility by the modulation of pacemaker activity through MAPKs and PLC pathways in the ICCs.
American Psychological Association (APA)
Hwang, Minwoo& Lee, Hee Jung& Song, Ho Joon& Kim, Byung Joo. 2013. Involvement of MAPKs and PLC Pathways in Modulation of Pacemaking Activity by So-Cheong-Ryong-Tang in Interstitial Cells of Cajal from Murine Small Intestine. The Scientific World Journal،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1033038
Modern Language Association (MLA)
Hwang, Minwoo…[et al.]. Involvement of MAPKs and PLC Pathways in Modulation of Pacemaking Activity by So-Cheong-Ryong-Tang in Interstitial Cells of Cajal from Murine Small Intestine. The Scientific World Journal No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-1033038
American Medical Association (AMA)
Hwang, Minwoo& Lee, Hee Jung& Song, Ho Joon& Kim, Byung Joo. Involvement of MAPKs and PLC Pathways in Modulation of Pacemaking Activity by So-Cheong-Ryong-Tang in Interstitial Cells of Cajal from Murine Small Intestine. The Scientific World Journal. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-1033038
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1033038