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Crystal Structure of a Conserved Hypothetical Protein MJ0927 from Methanocaldococcus jannaschii Reveals a Novel Quaternary Assembly in the Nif3 Family
Joint Authors
Chen, Sheng-Chia
Huang, Chi-Hung
Yang, Chia Shin
Kuan, Shu-Min
Chou, Shan-Ho
Chen, Yeh
Lin, Ching-Ting
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-08-28
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
A Nif3 family protein of Methanocaldococcus jannaschii, MJ0927, is highly conserved from bacteria to humans.
Although several structures of bacterial Nif3 proteins are known, no structure representing archaeal Nif3 has yet been reported.
The crystal structure of Methanocaldococcus jannaschii MJ0927 was determined at 2.47 Å resolution to understand the structural differences between the bacterial and archaeal Nif3 proteins.
Intriguingly, MJ0927 is found to adopt an unusual assembly comprising a trimer of dimers that forms a cage-like architecture.
Electrophoretic mobility-shift assays indicate that MJ0927 binds to both single-stranded and double-stranded DNA.
Structural analysis of MJ0927 reveals a positively charged region that can potentially explain its DNA-binding capability.
Taken together, these data suggest that MJ0927 adopts a novel quartenary architecture that could play various DNA-binding roles in Methanocaldococcus jannaschii.
American Psychological Association (APA)
Chen, Sheng-Chia& Huang, Chi-Hung& Yang, Chia Shin& Kuan, Shu-Min& Lin, Ching-Ting& Chou, Shan-Ho…[et al.]. 2014. Crystal Structure of a Conserved Hypothetical Protein MJ0927 from Methanocaldococcus jannaschii Reveals a Novel Quaternary Assembly in the Nif3 Family. BioMed Research International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1034392
Modern Language Association (MLA)
Chen, Sheng-Chia…[et al.]. Crystal Structure of a Conserved Hypothetical Protein MJ0927 from Methanocaldococcus jannaschii Reveals a Novel Quaternary Assembly in the Nif3 Family. BioMed Research International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1034392
American Medical Association (AMA)
Chen, Sheng-Chia& Huang, Chi-Hung& Yang, Chia Shin& Kuan, Shu-Min& Lin, Ching-Ting& Chou, Shan-Ho…[et al.]. Crystal Structure of a Conserved Hypothetical Protein MJ0927 from Methanocaldococcus jannaschii Reveals a Novel Quaternary Assembly in the Nif3 Family. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1034392
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1034392