Acute Toxicity Study of Zerumbone-Loaded Nanostructured Lipid Carrier on BALBc Mice Model
Joint Authors
How, Chee Wun
Rahman, Heshu Sulaiman
Abdul Samad, Nozlena
Ng, Kuan Beng
Anasamy, Theebaa
Chartrand, Max Stanley
Othman, Hemn Hassan
Namvar, Farideh
Andas, A. Reenaa Joys
Muhammad Nadzri, Nabilah
Rasedee, Abdullah
Yeap, Swee Keong
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-09-08
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Zerumbone- (ZER-) loaded nanostructure lipid carrier (NLC) (ZER-NLC) prepared for its antileukemia effect in vitro was evaluated for its toxicological effects by observing changes in the liver, kidney, spleen, lung, heart, and brain tissues, serum biochemical parameters, total haemogram, and bone marrow stem cells.
The acute toxicity study for ZER-NLC was conducted by orally treating BALB/c mice with a single dose with either water, olive oil, ZER, NLC, or ZER-NLC for 14 days.
The animals were observed for clinical and behavioral abnormalities, toxicological symptoms, feed consumption, and gross appearance.
The liver, kidney, heart, lung, spleen, and brain tissues were assessed histologically.
Total haemogram was counted by hemocytometry and microhematocrit reader.
Bone marrow examination in terms of cellular morphology was done by Wright staining with bone marrow smear.
Furthermore, serum biochemical parameters were determined spectrophotometrically.
Grossly all treated mice, their investigated tissues, serum biochemical parameters, total haemogram, and bone marrow were normal.
At oral doses of 100 and 200 mg/kg ZER-NLC there was no sign of toxicity or mortality in BALB/c mice.
This study suggests that the 50% lethal dose (LD50) of ZER-NLC is higher than 200 mg/kg, thus, safe by oral administration.
American Psychological Association (APA)
Rahman, Heshu Sulaiman& Rasedee, Abdullah& Othman, Hemn Hassan& Chartrand, Max Stanley& Namvar, Farideh& Yeap, Swee Keong…[et al.]. 2014. Acute Toxicity Study of Zerumbone-Loaded Nanostructured Lipid Carrier on BALBc Mice Model. BioMed Research International،Vol. 2014, no. 2014, pp.1-15.
https://search.emarefa.net/detail/BIM-1034499
Modern Language Association (MLA)
Rahman, Heshu Sulaiman…[et al.]. Acute Toxicity Study of Zerumbone-Loaded Nanostructured Lipid Carrier on BALBc Mice Model. BioMed Research International No. 2014 (2014), pp.1-15.
https://search.emarefa.net/detail/BIM-1034499
American Medical Association (AMA)
Rahman, Heshu Sulaiman& Rasedee, Abdullah& Othman, Hemn Hassan& Chartrand, Max Stanley& Namvar, Farideh& Yeap, Swee Keong…[et al.]. Acute Toxicity Study of Zerumbone-Loaded Nanostructured Lipid Carrier on BALBc Mice Model. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-15.
https://search.emarefa.net/detail/BIM-1034499
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1034499