Viola tricolor Induces Apoptosis in Cancer Cells and Exhibits Antiangiogenic Activity on Chicken Chorioallantoic Membrane
Joint Authors
Tayarani-Najaran, Zahra
Sadeghnia, Hamid Reza
Ghorbani Hesari, Taghi
Mousavi, Seyed Hadi
Ghorbani, Ahmad
Mortazavian, Seyed Mohsen
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-08-27
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
In the present study, the cytotoxic and apoptogenic properties of hydroalcoholic extract and ethyl acetate (EtOAc), n-butanol, and water fractions (0–800 μg/mL) of Viola tricolor were investigated in Neuro2a mouse neuroblastoma and MCF-7 human breast cancer cells.
In addition, antiangiogenic effect of EtOAc fraction was evaluated on chicken chorioallantoic membrane (CAM).
The quality of EtOAc fraction was also characterized using high performance liquid chromatography (HPLC) fingerprint.
Cytotoxicity assay revealed that EtOAc fraction was the most potent among all fractions with maximal effect on MCF-7 and minimal toxicity against normal murine fibroblast L929 cells.
Apoptosis induction by EtOAc fraction was confirmed by increased sub-G1 peak of propidium iodide (PI) stained cells.
This fraction triggered the apoptotic pathway by increased Bax/Bcl-2 ratio and cleaved caspase-3 level.
Moreover, treatment with EtOAc fraction significantly decreased the diameter of vessels on CAM, while the number of newly formed blood vessels was not suppressed significantly.
Analysis of quality of EtOAc fraction using HPLC fingerprint showed six major peaks with different retention times.
The results of the present study suggest that V.
tricolor has potential anticancer property by inducing apoptosis and inhibiting angiogenesis.
American Psychological Association (APA)
Sadeghnia, Hamid Reza& Ghorbani Hesari, Taghi& Mortazavian, Seyed Mohsen& Mousavi, Seyed Hadi& Tayarani-Najaran, Zahra& Ghorbani, Ahmad. 2014. Viola tricolor Induces Apoptosis in Cancer Cells and Exhibits Antiangiogenic Activity on Chicken Chorioallantoic Membrane. BioMed Research International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1034514
Modern Language Association (MLA)
Sadeghnia, Hamid Reza…[et al.]. Viola tricolor Induces Apoptosis in Cancer Cells and Exhibits Antiangiogenic Activity on Chicken Chorioallantoic Membrane. BioMed Research International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1034514
American Medical Association (AMA)
Sadeghnia, Hamid Reza& Ghorbani Hesari, Taghi& Mortazavian, Seyed Mohsen& Mousavi, Seyed Hadi& Tayarani-Najaran, Zahra& Ghorbani, Ahmad. Viola tricolor Induces Apoptosis in Cancer Cells and Exhibits Antiangiogenic Activity on Chicken Chorioallantoic Membrane. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1034514
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1034514