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Changes in ADMADDAH Pathway after Hepatic IschemiaReperfusion Injury in Rats: The Role of Bile
Joint Authors
Ferrigno, Andrea
Berardo, Clarissa
Bianchi, Alberto
Rizzo, Vittoria
Richelmi, Plinio
Vairetti, Mariapia
Di Pasqua, Laura G.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-08-26
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
We investigated the effects of hepatic ischemia/reperfusion (I/R) injury on asymmetric dimethylarginine (ADMA, a nitric oxide synthase inhibitor), protein methyltransferase (PRMT) and dimethylarginine dimethylaminohydrolase (DDAH) (involved, resp., in ADMA synthesis and degradation), and the cationic transporter (CAT).
Male Wistar rats were subjected to 30 or 60 min hepatic ischemia followed by 60 min reperfusion.
ADMA levels in serum and bile were determined.
Tissue ADMA, DDAH activity, DDAH-1 and CAT-2 protein, DDAH-1 and PRMT-1 mRNA expression, GSH/GSSG, ROS production, and lipid peroxidation were detected.
ADMA was found in bile.
I/R increased serum and bile ADMA levels while an intracellular decrease was detected after 60 min ischemia.
Decreased DDAH activity, mRNA, and protein expression were observed at the end of reperfusion.
No significant difference was observed in GSH/GSSG, ROS, lipid peroxidation, and CAT-2; a decrease in PRMT-1 mRNA expression was found after I/R.
Liver is responsible for the biliary excretion of ADMA, as documented here for the first time, and I/R injury is associated with an oxidative stress-independent alteration in DDAH activity.
These data are a step forward in the understanding of the pathways that regulate serum, tissue, and biliary levels of ADMA in which DDAH enzyme plays a crucial role.
American Psychological Association (APA)
Ferrigno, Andrea& Rizzo, Vittoria& Bianchi, Alberto& Di Pasqua, Laura G.& Berardo, Clarissa& Richelmi, Plinio…[et al.]. 2014. Changes in ADMADDAH Pathway after Hepatic IschemiaReperfusion Injury in Rats: The Role of Bile. BioMed Research International،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1034515
Modern Language Association (MLA)
Ferrigno, Andrea…[et al.]. Changes in ADMADDAH Pathway after Hepatic IschemiaReperfusion Injury in Rats: The Role of Bile. BioMed Research International No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-1034515
American Medical Association (AMA)
Ferrigno, Andrea& Rizzo, Vittoria& Bianchi, Alberto& Di Pasqua, Laura G.& Berardo, Clarissa& Richelmi, Plinio…[et al.]. Changes in ADMADDAH Pathway after Hepatic IschemiaReperfusion Injury in Rats: The Role of Bile. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1034515
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1034515