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Assessment of Tumor Cells in a Mouse Model of Diffuse Infiltrative Glioma by Raman Spectroscopy
Joint Authors
Ohka, Fumiharu
Wakabayashi, Toshihiko
Tanahashi, Kuniaki
Momota, Hiroyuki
Kato, Akira
Motomura, Kazuya
Watabe, Naoki
Muraishi, Shuichi
Nakahara, Hitoshi
Saito, Yahachi
Takeuchi, Ichiro
Natsume, Atsushi
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-08-27
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Glioma of infiltrative nature is challenging for surgeons to achieve tumor-specific and maximal resection.
Raman spectroscopy provides structural information on the targeted materials as vibrational shifts.
We utilized Raman spectroscopy to distinguish invasive tumors from normal tissues.
Spectra obtained from replication-competent avian sarcoma-(RCAS-) based infiltrative glioma cells and glioma tissues (resembling low-grade human glioma) were compared with those obtained from normal mouse astrocytes and normal tissues.
In cell analysis, the spectra at 950–1000, 1030, 1050–1100, 1120–1130, 1120–1200, 1200–1300, 1300–1350, and 1450 cm−1 were significantly higher in infiltrative glioma cells than in normal astrocytes.
In brain tissue analysis, the spectra at 1030, 1050–1100, and 1200–1300 cm−1 were significantly higher in infiltrative glioma tissues than in normal brain tissues.
These spectra reflect the structures of proteins, lipids, and DNA content.
The sensitivity and specificity to predict glioma cells by distinguishing normal cells were 98.3% and 75.0%, respectively.
Principal component analysis elucidated the significance of spectral difference between tumor tissues and normal tissues.
It is possible to distinguish invasive tumors from normal tissues by using Raman spectroscopy.
American Psychological Association (APA)
Tanahashi, Kuniaki& Natsume, Atsushi& Ohka, Fumiharu& Momota, Hiroyuki& Kato, Akira& Motomura, Kazuya…[et al.]. 2014. Assessment of Tumor Cells in a Mouse Model of Diffuse Infiltrative Glioma by Raman Spectroscopy. BioMed Research International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1034585
Modern Language Association (MLA)
Tanahashi, Kuniaki…[et al.]. Assessment of Tumor Cells in a Mouse Model of Diffuse Infiltrative Glioma by Raman Spectroscopy. BioMed Research International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1034585
American Medical Association (AMA)
Tanahashi, Kuniaki& Natsume, Atsushi& Ohka, Fumiharu& Momota, Hiroyuki& Kato, Akira& Motomura, Kazuya…[et al.]. Assessment of Tumor Cells in a Mouse Model of Diffuse Infiltrative Glioma by Raman Spectroscopy. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1034585
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1034585