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Effects of Chinese Medicine Tong xinluo on Diabetic Nephropathy via Inhibiting TGF-β1-Induced Epithelial-to-Mesenchymal Transition
Joint Authors
Wang, Jin-Yang
Gao, Yan-Bin
Li, Jiaoyang
Zhou, Shengnan
Zhao, Xuan
Xu, Liping
Zhang, Haiyan
Zou, Da-Wei
Zhu, Zhi-Yao
Zhang, Na
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-17
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Diabetic nephropathy (DN) is a major cause of chronic kidney failure and characterized by interstitial and glomeruli fibrosis.
Epithelial-to-mesenchymal transition (EMT) plays an important role in the pathogenesis of DN.
Tong xinluo (TXL), a Chinese herbal compound, has been used in China with established therapeutic efficacy in patients with DN.
To investigate the molecular mechanism of TXL improving DN, KK-Ay mice were selected as models for the evaluation of pathogenesis and treatment in DN.
In vitro, TGF-β1 was used to induce EMT.
Western blot (WB), immunofluorescence staining, and real-time polymerase chain reaction (RT-PCR) were applied to detect the changes of EMT markers in vivo and in vitro, respectively.
Results showed the expressions of TGF-β1 and its downstream proteins smad3/p-smad3 were greatly reduced in TXL group; meantime, TXL restored the expression of smad7.
As a result, the expressions of collagen IV (Col IV) and fibronectin (FN) were significantly decreased in TXL group.
In vivo, 24 h-UAER (24-hour urine albumin excretion ratio) and BUN (blood urea nitrogen) were decreased and Ccr (creatinine clearance ratio) was increased in TXL group compared with DN group.
In summary, the present study demonstrates that TXL successfully inhibits TGF-β1-induced epithelial-to-mesenchymal transition in DN, which may account for the therapeutic efficacy in TXL-mediated renoprotection.
American Psychological Association (APA)
Zhang, Na& Gao, Yan-Bin& Zou, Da-Wei& Wang, Jin-Yang& Li, Jiaoyang& Zhou, Shengnan…[et al.]. 2014. Effects of Chinese Medicine Tong xinluo on Diabetic Nephropathy via Inhibiting TGF-β1-Induced Epithelial-to-Mesenchymal Transition. Evidence-Based Complementary and Alternative Medicine،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1035096
Modern Language Association (MLA)
Zhang, Na…[et al.]. Effects of Chinese Medicine Tong xinluo on Diabetic Nephropathy via Inhibiting TGF-β1-Induced Epithelial-to-Mesenchymal Transition. Evidence-Based Complementary and Alternative Medicine No. 2014 (2014), pp.1-12.
https://search.emarefa.net/detail/BIM-1035096
American Medical Association (AMA)
Zhang, Na& Gao, Yan-Bin& Zou, Da-Wei& Wang, Jin-Yang& Li, Jiaoyang& Zhou, Shengnan…[et al.]. Effects of Chinese Medicine Tong xinluo on Diabetic Nephropathy via Inhibiting TGF-β1-Induced Epithelial-to-Mesenchymal Transition. Evidence-Based Complementary and Alternative Medicine. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1035096
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1035096