Effect of Wasabi Component 6-(Methylsulfinyl)hexyl Isothiocyanate and Derivatives on Human Pancreatic Cancer Cells
Joint Authors
Liao, Hui-Fen
Huang, Yu-Chuen
Chen, Yu-Jen
Tsai, Tung-Hu
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-01-20
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
The naturally occurring compound 6-(methylsulfinyl)hexyl isothiocyanate (6-MITC) was isolated from Wasabia japonica (Wasabi), a pungent spice used in Japanese food worldwide.
The synthetic derivatives 6-(methylsulfenyl)hexyl isothiocyanate (I7447) and 6-(methylsulfonyl)hexyl isothiocyanate (I7557) are small molecule compounds derived from 6-MITC.
This study aimed to evaluate the effect of these compounds on human pancreatic cancer cells.
Human pancreatic cancer cell lines PANC-1 and BxPC-3 were used to perform an MTT assay for cell viability and Liu’s stain for morphological observation.
The cell cycle was analyzed by DNA histogram.
Aldehyde dehydrogenase (ALDH) activity was used as a marker for cancer stem cells (CSC).
Western blotting was performed for the expression of proteins related to CSC signaling.
The results showed that compounds 6-MITC and I7557, but not I7447, inhibited viability of both PANC-1 and BxPC-3 cells.
Morphological observation showed mitotic arrest and apoptosis in 6-MITC- and I7557-treated cells.
These two compounds induced G2/M phase arrest and hypoploid population.
Percentages of ALDH-positive PANC-1 cells were markedly reduced by 6-MITC and I7557 treatment.
The expression of CSC signaling molecule SOX2, but not NOTCH1, ABCG2, Sonic hedgehog, or OCT4, was inhibited by 6-MITC and I7557.
In conclusion, wasabi compounds 6-MITC and I7557 may possess activity against the growth and CSC phenotypes of human pancreatic cancer cells.
American Psychological Association (APA)
Chen, Yu-Jen& Huang, Yu-Chuen& Tsai, Tung-Hu& Liao, Hui-Fen. 2014. Effect of Wasabi Component 6-(Methylsulfinyl)hexyl Isothiocyanate and Derivatives on Human Pancreatic Cancer Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1035225
Modern Language Association (MLA)
Chen, Yu-Jen…[et al.]. Effect of Wasabi Component 6-(Methylsulfinyl)hexyl Isothiocyanate and Derivatives on Human Pancreatic Cancer Cells. Evidence-Based Complementary and Alternative Medicine No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-1035225
American Medical Association (AMA)
Chen, Yu-Jen& Huang, Yu-Chuen& Tsai, Tung-Hu& Liao, Hui-Fen. Effect of Wasabi Component 6-(Methylsulfinyl)hexyl Isothiocyanate and Derivatives on Human Pancreatic Cancer Cells. Evidence-Based Complementary and Alternative Medicine. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1035225
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1035225