Effect of Garden Cress Seeds Powder and Its Alcoholic Extract on the Metabolic Activity of CYP2D6 and CYP3A4
Joint Authors
Alkharfy, Khalid M.
Al-Jenoobi, F. I.
Al-Suwayeh, S. A.
Alam, Mohd Aftab
Al-Mohizea, Abdullah M.
Al-Thukair, Areej A.
Abbas, Fawkeya A.
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-10
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
The powder and alcoholic extract of dried seeds of garden cress were investigated for their effect on metabolic activity of CYP2D6 and CYP3A4 enzymes.
In vitro and clinical studies were conducted on human liver microsomes and healthy human subjects, respectively.
Dextromethorphan was used as a common marker for measuring metabolic activity of CYP2D6 and CYP3A4 enzymes.
In in vitro studies, microsomes were incubated with NADPH in presence and absence of different concentrations of seeds extract.
Clinical investigations were performed in two phases.
In phase I, six healthy female volunteers were administered a single dose of dextromethorphan and in phase II volunteers were treated with seeds powder for seven days and dextromethorphan was administered with last dose.
The O-demethylated and N-demethylated metabolites of dextromethorphan were measured as dextrorphan (DOR) and 3-methoxymorphinan (3-MM), respectively.
Observations suggested that garden cress inhibits the formation of DOR and 3-MM metabolites.
This inhibition of metabolite level was attributed to the inhibition of CYP2D6 and CYP3A4 activity.
Garden cress decreases the level of DOR and 3-MM in urine and significantly increases the urinary metabolic ratio of DEX/DOR and DEX/3-MM.
The findings suggested that garden cress seeds powder and ethanolic extract have the potential to interact with CYP2D6 and CYP3A4 substrates.
American Psychological Association (APA)
Al-Jenoobi, F. I.& Al-Thukair, Areej A.& Alam, Mohd Aftab& Abbas, Fawkeya A.& Al-Mohizea, Abdullah M.& Alkharfy, Khalid M.…[et al.]. 2014. Effect of Garden Cress Seeds Powder and Its Alcoholic Extract on the Metabolic Activity of CYP2D6 and CYP3A4. Evidence-Based Complementary and Alternative Medicine،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1035313
Modern Language Association (MLA)
Al-Jenoobi, F. I.…[et al.]. Effect of Garden Cress Seeds Powder and Its Alcoholic Extract on the Metabolic Activity of CYP2D6 and CYP3A4. Evidence-Based Complementary and Alternative Medicine No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-1035313
American Medical Association (AMA)
Al-Jenoobi, F. I.& Al-Thukair, Areej A.& Alam, Mohd Aftab& Abbas, Fawkeya A.& Al-Mohizea, Abdullah M.& Alkharfy, Khalid M.…[et al.]. Effect of Garden Cress Seeds Powder and Its Alcoholic Extract on the Metabolic Activity of CYP2D6 and CYP3A4. Evidence-Based Complementary and Alternative Medicine. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1035313
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1035313