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Effects of 6-Hydroxyflavone on Osteoblast Differentiation in MC3T3-E1 Cells
Joint Authors
Lai, Chien-Hung
Wu, Yu-Wei
Yeh, Shauh-Der
Lin, Yu-Hsaing
Tsai, Yu-Hui
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-26
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Osteoblast differentiation plays an essential role in bone integrity.
Isoflavones and some flavonoids are reported to have osteogenic activity and potentially possess the ability to treat osteoporosis.
However, limited information concerning the osteogenic characteristics of hydroxyflavones is available.
This study investigates the effects of various hydroxyflavones on osteoblast differentiation in MC3T3-E1 cells.
The results showed that 6-hydroxyflavone (6-OH-F) and 7-hydroxyflavone (7-OH-F) stimulated ALP activity.
However, baicalein and luteolin inhibited ALP activity and flavone showed no effect.
Up to 50 μM of each compound was used for cytotoxic effects study; flavone, 6-OH-F, and 7-OH-F had no cytotoxicity on MC3T3-E1 cells.
Moreover, 6-OH-F activated AKT and serine/threonine kinases (also known as protein kinase B or PKB), extracellular signal-regulated kinases (ERK 1/2), and the c-Jun N-terminal kinase (JNK) signaling pathways.
On the other hand, 7-OH-F promoted osteoblast differentiation mainly by activating ERK 1/ 2 signaling pathways.
Finally, after 5 weeks of 6-OH-F induction, MC3T3-E1 cells showed a significant increase in the calcein staining intensity relative to merely visible mineralization observed in cells cultured in the osteogenic medium only.
These results suggested that 6-OH-F could activate AKT, ERK 1/2, and JNK signaling pathways to effectively promote osteoblastic differentiation.
American Psychological Association (APA)
Lai, Chien-Hung& Wu, Yu-Wei& Yeh, Shauh-Der& Lin, Yu-Hsaing& Tsai, Yu-Hui. 2014. Effects of 6-Hydroxyflavone on Osteoblast Differentiation in MC3T3-E1 Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1035455
Modern Language Association (MLA)
Lai, Chien-Hung…[et al.]. Effects of 6-Hydroxyflavone on Osteoblast Differentiation in MC3T3-E1 Cells. Evidence-Based Complementary and Alternative Medicine No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-1035455
American Medical Association (AMA)
Lai, Chien-Hung& Wu, Yu-Wei& Yeh, Shauh-Der& Lin, Yu-Hsaing& Tsai, Yu-Hui. Effects of 6-Hydroxyflavone on Osteoblast Differentiation in MC3T3-E1 Cells. Evidence-Based Complementary and Alternative Medicine. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1035455
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1035455