Enucleation-Induced Rat Adrenal Gland Regeneration: Expression Profile of Selected Genes Involved in Control of Adrenocortical Cell Proliferation
Joint Authors
Ruciński, Marcin
Malendowicz, Ludwik K.
Tyczewska, Marianna
Ziolkowska, Agnieszka
Szyszka, Marta
Trejter, Marcin
Hochol-Molenda, Anna
Nowak, Krzysztof W.
Source
International Journal of Endocrinology
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-11-06
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Enucleation-induced adrenal regeneration is a highly controlled process; however, only some elements involved in this process have been recognized.
Therefore, we performed studies on regenerating rat adrenals.
Microarray RNA analysis and QPCR revealed that enucleation resulted in a rapid elevation of expression of genes involved in response to wounding, defense response, and in immunological processes.
Factors encoded by these genes obscure possible priming effects of various cytokines on initiation of regeneration.
In regenerating adrenals we identified over 100 up- or downregulated genes involved in adrenocortical cell proliferation.
The changes were most significant at days 2-3 after enucleation and their number decreased during regeneration.
For example, expression analysis revealed a notable upregulation of the growth arrest gene, Gadd45, only 24 hours after surgery while expression of cyclin B1 and Cdk1 genes was notably elevated between days 1–8 of regeneration.
These changes were accompanied by changes in expression levels of numerous growth factors and immediate-early transcription factors genes.
Despite notable differences in mechanisms of adrenal and liver regeneration, in regenerating adrenals we identified genes, the expression of which is well recognized in regenerating liver.
Thus, it seems legitimate to suggest that, in the rat, the general model of liver and adrenal regeneration demonstrate some degree of similarity.
American Psychological Association (APA)
Tyczewska, Marianna& Ruciński, Marcin& Ziolkowska, Agnieszka& Szyszka, Marta& Trejter, Marcin& Hochol-Molenda, Anna…[et al.]. 2014. Enucleation-Induced Rat Adrenal Gland Regeneration: Expression Profile of Selected Genes Involved in Control of Adrenocortical Cell Proliferation. International Journal of Endocrinology،Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-1036413
Modern Language Association (MLA)
Tyczewska, Marianna…[et al.]. Enucleation-Induced Rat Adrenal Gland Regeneration: Expression Profile of Selected Genes Involved in Control of Adrenocortical Cell Proliferation. International Journal of Endocrinology No. 2014 (2014), pp.1-13.
https://search.emarefa.net/detail/BIM-1036413
American Medical Association (AMA)
Tyczewska, Marianna& Ruciński, Marcin& Ziolkowska, Agnieszka& Szyszka, Marta& Trejter, Marcin& Hochol-Molenda, Anna…[et al.]. Enucleation-Induced Rat Adrenal Gland Regeneration: Expression Profile of Selected Genes Involved in Control of Adrenocortical Cell Proliferation. International Journal of Endocrinology. 2014. Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-1036413
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1036413