Liraglutide Exerts Antidiabetic Effect via PTP1B and PI3KAkt2 Signaling Pathway in Skeletal Muscle of KKAy Mice
Joint Authors
Ji, Wenjun
Chen, Xinlin
Lv, Juan
Wang, Meng
Ren, Shuting
Yuan, Bingxiang
Wang, Bing
Chen, Lina
Source
International Journal of Endocrinology
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-08-11
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Background.
Liraglutide (a glucagon-like peptide 1 analog) was used for the treatment of type 2 diabetes (T2DM) which could produce glucose-dependent insulin secretion.
Aim.
The aim was to investigate whether liraglutide could improve myofibril and mitochondria injury in skeletal muscle and the mechanisms in diabetic KKAy mice.
Method.
We divided the male KKAy mice into 2 groups: liraglutide group (250 μg/kg/day liraglutide subcutaneous injection) and model group; meanwhile, the male C57BL/6J mice were considered as the control.
After 6 weeks, the ultrastructure of skeletal muscle was observed by electron microscope.
The gene expressions of protein tyrosine phosphatase 1B (PTP1B), phosphatidylinositol 3-kinase (PI3K), and glucose transporter type 4 (GLUT4) were determined by real-time PCR.
The protein levels of the above molecules and phospho-Akt2 (p-Akt2) were measured by Western blot.
Results.
Liraglutide significantly ameliorated the injury of mitochondria by increasing the number (+441%) and the area (+113%) of mitochondria and mitochondrial area/100 µm2 (+396%) in skeletal muscle of KKAy mice.
The results of real-time PCR and Western blot showed that liraglutide downregulated PTP1B while it upregulated PI3K and GLUT4 (P<0.01).
The protein level of p-Akt2/Akt2 was also increased (P<0.01).
Conclusion.
These results revealed that liraglutide could improve myofibril and mitochondria injury in skeletal muscle against T2DM via PTP1B and PI3K/Akt2 signaling pathway.
American Psychological Association (APA)
Ji, Wenjun& Chen, Xinlin& Lv, Juan& Wang, Meng& Ren, Shuting& Yuan, Bingxiang…[et al.]. 2014. Liraglutide Exerts Antidiabetic Effect via PTP1B and PI3KAkt2 Signaling Pathway in Skeletal Muscle of KKAy Mice. International Journal of Endocrinology،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1036461
Modern Language Association (MLA)
Ji, Wenjun…[et al.]. Liraglutide Exerts Antidiabetic Effect via PTP1B and PI3KAkt2 Signaling Pathway in Skeletal Muscle of KKAy Mice. International Journal of Endocrinology No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1036461
American Medical Association (AMA)
Ji, Wenjun& Chen, Xinlin& Lv, Juan& Wang, Meng& Ren, Shuting& Yuan, Bingxiang…[et al.]. Liraglutide Exerts Antidiabetic Effect via PTP1B and PI3KAkt2 Signaling Pathway in Skeletal Muscle of KKAy Mice. International Journal of Endocrinology. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1036461
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1036461