Gene Expression Profiling of the Paracrine Effects of Uterine Natural Killer Cells on Human Endometrial Epithelial Cells
Joint Authors
Gong, Xin
Chen, Zhenzhen
Liu, Yanxia
Lu, Qiudan
Jin, Zhe
Source
International Journal of Endocrinology
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-26
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
The endometrium contains a population of immune cells that undergo changes during implantation and pregnancy.
The majority of these cells are uterine natural killer (uNK) cells; however, it is unclear how these cells interact with endometrial epithelial cells.
Therefore, we investigated the paracrine effects of the uNK cell-secretion medium on the gene expression profile of endometrial epithelial cells in vitro through microarray analysis.
Our results, which were verified by qRT-PCR and western blot, revealed that soluble factors from uNK cells alter the gene expression profiles of epithelial cells.
The upregulated genes included interleukin-15 (IL-15) and interleukin-15 receptor alpha (IL-15RA), which result in a loop that stimulates uNK cell proliferation.
In addition, vascular endothelial growth factor C (VEGF-C) and chemokine (C-X-C motif) ligand 10 (CXCL-10) were also determined to be upregulated in epithelial cells, which suggests that uNK cells work synergistically with epithelial cells to support implantation and pregnancy.
In addition, oriental herbal medicines have been used to treat infertility since ancient times; however, we failed to find that Zi Dan Yin can regulate these endometrial paracrine effects.
American Psychological Association (APA)
Gong, Xin& Chen, Zhenzhen& Liu, Yanxia& Lu, Qiudan& Jin, Zhe. 2014. Gene Expression Profiling of the Paracrine Effects of Uterine Natural Killer Cells on Human Endometrial Epithelial Cells. International Journal of Endocrinology،Vol. 2014, no. 2014, pp.1-15.
https://search.emarefa.net/detail/BIM-1036488
Modern Language Association (MLA)
Gong, Xin…[et al.]. Gene Expression Profiling of the Paracrine Effects of Uterine Natural Killer Cells on Human Endometrial Epithelial Cells. International Journal of Endocrinology No. 2014 (2014), pp.1-15.
https://search.emarefa.net/detail/BIM-1036488
American Medical Association (AMA)
Gong, Xin& Chen, Zhenzhen& Liu, Yanxia& Lu, Qiudan& Jin, Zhe. Gene Expression Profiling of the Paracrine Effects of Uterine Natural Killer Cells on Human Endometrial Epithelial Cells. International Journal of Endocrinology. 2014. Vol. 2014, no. 2014, pp.1-15.
https://search.emarefa.net/detail/BIM-1036488
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1036488