Facile, High Quality Sequencing of Bacterial Genomes from Small Amounts of DNA
Joint Authors
Vuyisich, Momchilo
Arefin, Ayesha
Davenport, Karen
Feng, Shihai
Gleasner, Cheryl
McMurry, Kim
Parson-Quintana, Beverly
Price, Jennifer
Scholz, Matthew
Chain, Patrick
Source
International Journal of Genomics
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-11-12
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Sequencing bacterial genomes has traditionally required large amounts of genomic DNA (~1 μ g).
There have been few studies to determine the effects of the input DNA amount or library preparation method on the quality of sequencing data.
Several new commercially available library preparation methods enable shotgun sequencing from as little as 1 ng of input DNA.
In this study, we evaluated the NEBNext Ultra library preparation reagents for sequencing bacterial genomes.
We have evaluated the utility of NEBNext Ultra for resequencing and de novo assembly of four bacterial genomes and compared its performance with the TruSeq library preparation kit.
The NEBNext Ultra reagents enable high quality resequencing and de novo assembly of a variety of bacterial genomes when using 100 ng of input genomic DNA.
For the two most challenging genomes (Burkholderia spp.), which have the highest GC content and are the longest, we also show that the quality of both resequencing and de novo assembly is not decreased when only 10 ng of input genomic DNA is used.
American Psychological Association (APA)
Vuyisich, Momchilo& Arefin, Ayesha& Davenport, Karen& Feng, Shihai& Gleasner, Cheryl& McMurry, Kim…[et al.]. 2014. Facile, High Quality Sequencing of Bacterial Genomes from Small Amounts of DNA. International Journal of Genomics،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1036691
Modern Language Association (MLA)
Vuyisich, Momchilo…[et al.]. Facile, High Quality Sequencing of Bacterial Genomes from Small Amounts of DNA. International Journal of Genomics No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1036691
American Medical Association (AMA)
Vuyisich, Momchilo& Arefin, Ayesha& Davenport, Karen& Feng, Shihai& Gleasner, Cheryl& McMurry, Kim…[et al.]. Facile, High Quality Sequencing of Bacterial Genomes from Small Amounts of DNA. International Journal of Genomics. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1036691
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1036691