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Circulating MicroRNAs in Plasma of Hepatitis B e Antigen Positive Children Reveal Liver-Specific Target Genes
Joint Authors
Winther, Thilde Nordmann
Jacobsen, Kari Stougaard
Mirza, Aashiq Hussain
Heiberg, Ida Louise
Bang-Berthelsen, Claus Heiner
Pociot, Flemming
Hogh, Birthe
Source
International Journal of Hepatology
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-12-17
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Background and Aim.
Hepatitis B e antigen positive (HBeAg-positive) children are at high risk of severe complications such as hepatocellular carcinoma and cirrhosis.
Liver damage is caused by the host immune response to infected hepatocytes, and we hypothesise that specific microRNAs play a role in this complex interaction between virus and host.
The study aimed to identify microRNAs with aberrant plasma expressions in HBeAg-positive children and with liver-specific target genes.
Methods.
By revisiting our previous screen of microRNA plasma levels in HBeAg-positive and HBeAg-negative children with chronic hepatitis B (CHB) and in healthy controls, candidate microRNAs with aberrant plasma expressions in HBeAg-positive children were identified.
MicroRNAs targeting liver-specific genes were selected based on bioinformatics analysis and validated by qRT-PCR using plasma samples from 34 HBeAg-positive, 26 HBeAg-negative, and 60 healthy control children.
Results.
Thirteen microRNAs showed aberrant plasma expressions in HBeAg-positive children and targeted liver-specific genes.
In particular, three microRNAs were upregulated and one was downregulated in HBeAg-positive children compared to HBeAg-negative and healthy control children, which showed equal levels.
Conclusion.
The identified microRNAs might impact the progression of CHB in children.
Functional studies are warranted, however, to elucidate the microRNAs’ role in the immunopathogenesis of childhood CHB.
American Psychological Association (APA)
Winther, Thilde Nordmann& Jacobsen, Kari Stougaard& Mirza, Aashiq Hussain& Heiberg, Ida Louise& Bang-Berthelsen, Claus Heiner& Pociot, Flemming…[et al.]. 2014. Circulating MicroRNAs in Plasma of Hepatitis B e Antigen Positive Children Reveal Liver-Specific Target Genes. International Journal of Hepatology،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1036710
Modern Language Association (MLA)
Winther, Thilde Nordmann…[et al.]. Circulating MicroRNAs in Plasma of Hepatitis B e Antigen Positive Children Reveal Liver-Specific Target Genes. International Journal of Hepatology No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1036710
American Medical Association (AMA)
Winther, Thilde Nordmann& Jacobsen, Kari Stougaard& Mirza, Aashiq Hussain& Heiberg, Ida Louise& Bang-Berthelsen, Claus Heiner& Pociot, Flemming…[et al.]. Circulating MicroRNAs in Plasma of Hepatitis B e Antigen Positive Children Reveal Liver-Specific Target Genes. International Journal of Hepatology. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1036710
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1036710