Extraction and Bioactivity Analysis of Major Flavones Compounds from Scutellaria baicalensis Using In Vitro Assay and Online Screening HPLC-ABTS System
Joint Authors
Ma, Jin Yeul
Lee, Kwang Jin
Oh, You-Chang
Song, Na-Young
Jung, Pil Mun
Kim, Taesoo
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-08-31
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The extraction efficiency of a number of solvent compositions for the improvement of bioactive compounds yield from S.
baicalensis has been investigated.
Also, free radical scavengers in the glycoside baicalin (BG), wogonoside (WG), aglycon baicalein (B), and wogonin (W) compounds of S.
baicalensis were screened, identified, and quantified using coupled offline ABTS and online screening HPLC-ABTS assay.
Increasing ethanol content fractions resulted in decreased extract yield of bioactive compounds.
In this case, the best yield of 37.01 mg/g in BG, WG, B, and W compounds was obtained by a dipping method with an extraction time of 4 h.
In addition, the yield (43.05%) and IC50 (34.04 μg/mL) determined through ABTS assay of the 60% aqueous ethanol extract were the most satisfactory of all solvent solutions tested.
This result shows that an online screening HPLC-ABTS assay can be a powerful technique for the rapid characterization of bioactivity compounds in plant extracts.
Moreover, their anti-inflammatory activities were evaluated via analyzed inhibitory effect on NO and inflammatory cytokine production.
Furthermore, WG and W exhibited the strong inhibitory effects on inflammatory mediator production including NO, IL-6, and IL-1β in LPS-stimulated RAW 264.7 macrophages.
American Psychological Association (APA)
Lee, Kwang Jin& Jung, Pil Mun& Oh, You-Chang& Song, Na-Young& Kim, Taesoo& Ma, Jin Yeul. 2014. Extraction and Bioactivity Analysis of Major Flavones Compounds from Scutellaria baicalensis Using In Vitro Assay and Online Screening HPLC-ABTS System. Journal of Analytical Methods in Chemistry،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1039832
Modern Language Association (MLA)
Lee, Kwang Jin…[et al.]. Extraction and Bioactivity Analysis of Major Flavones Compounds from Scutellaria baicalensis Using In Vitro Assay and Online Screening HPLC-ABTS System. Journal of Analytical Methods in Chemistry No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1039832
American Medical Association (AMA)
Lee, Kwang Jin& Jung, Pil Mun& Oh, You-Chang& Song, Na-Young& Kim, Taesoo& Ma, Jin Yeul. Extraction and Bioactivity Analysis of Major Flavones Compounds from Scutellaria baicalensis Using In Vitro Assay and Online Screening HPLC-ABTS System. Journal of Analytical Methods in Chemistry. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1039832
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1039832