Generating and Reversing Chronic Wounds in Diabetic Mice by Manipulating Wound Redox Parameters
Joint Authors
Martins-Green, Manuela
Dhall, Sandeep
Do, Danh C.
Garcia, Monika
Kim, Jane
Mirebrahim, Seyed H.
Lyubovitsky, Julia
Lonardi, Stefano
Nothnagel, Eugene A.
Schiller, Neal
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-18, 18 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-12-23
Country of Publication
Egypt
No. of Pages
18
Main Subjects
Abstract EN
By 2025, more than 500 M people worldwide will suffer from diabetes; 125 M will develop foot ulcer(s) and 20 M will undergo an amputation, creating a major health problem.
Understanding how these wounds become chronic will provide insights to reverse chronicity.
We hypothesized that oxidative stress (OS) in wounds is a critical component for generation of chronicity.
We used the db/db mouse model of impaired healing and inhibited, at time of injury, two major antioxidant enzymes, catalase and glutathione peroxidase, creating high OS in the wounds.
This was necessary and sufficient to trigger wounds to become chronic.
The wounds initially contained a polymicrobial community that with time selected for specific biofilm-forming bacteria.
To reverse chronicity we treated the wounds with the antioxidants α-tocopherol and N-acetylcysteine and found that OS was highly reduced, biofilms had increased sensitivity to antibiotics, and granulation tissue was formed with proper collagen deposition and remodeling.
We show for the first time generation of chronic wounds in which biofilm develops spontaneously, illustrating importance of early and continued redox imbalance coupled with the presence of biofilm in development of wound chronicity.
This model will help decipher additional mechanisms and potentially better diagnosis of chronicity and treatment of human chronic wounds.
American Psychological Association (APA)
Dhall, Sandeep& Do, Danh C.& Garcia, Monika& Kim, Jane& Mirebrahim, Seyed H.& Lyubovitsky, Julia…[et al.]. 2014. Generating and Reversing Chronic Wounds in Diabetic Mice by Manipulating Wound Redox Parameters. Journal of Diabetes Research،Vol. 2014, no. 2014, pp.1-18.
https://search.emarefa.net/detail/BIM-1040311
Modern Language Association (MLA)
Dhall, Sandeep…[et al.]. Generating and Reversing Chronic Wounds in Diabetic Mice by Manipulating Wound Redox Parameters. Journal of Diabetes Research No. 2014 (2014), pp.1-18.
https://search.emarefa.net/detail/BIM-1040311
American Medical Association (AMA)
Dhall, Sandeep& Do, Danh C.& Garcia, Monika& Kim, Jane& Mirebrahim, Seyed H.& Lyubovitsky, Julia…[et al.]. Generating and Reversing Chronic Wounds in Diabetic Mice by Manipulating Wound Redox Parameters. Journal of Diabetes Research. 2014. Vol. 2014, no. 2014, pp.1-18.
https://search.emarefa.net/detail/BIM-1040311
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1040311