Appropriate Development of the Liver Treg Compartment Is Modulated by the Microbiota and Requires TGF-β and MyD88
Joint Authors
Maria, Ann
English, Kathryn A.
Gorham, James D.
Source
Journal of Immunology Research
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-08-07
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Neither the early postnatal development of the liver Treg compartment nor the factors that regulate its development has been characterized.
We compared the early developmental patterns of Treg cell accumulation in murine liver, thymus, and spleen.
A FoxP3EGFP reporter mouse was employed to identify Treg cells.
Mononuclear cells were isolated from organs postnatally, stained for CD4, and examined by flow cytometry to enumerate FoxP3+ CD4 hi cells.
To assess roles for TGF- β 1 , MyD88, and TLR2, gene-specific knockout pups were generated from heterozygous breeders.
To test the role of commensal bacteria, pregnant dams were administered antibiotics during gestation and after parturition.
The pattern of appearance of Treg cells differed in liver, spleen, and thymus.
Notably, at 1-2 weeks, the frequency of CD4 hi FoxP3+ T cells in liver exceeded that in spleen by 1.5- to 2-fold.
The relative increase in liver Treg frequency was transient and was dependent upon TGF- β 1 and MyD88, but not TLR2, and was abrogated by antibiotic treatment.
A relative increase in liver Treg frequency occurs approximately 1-2 weeks after parturition that appears to be driven by colonization of the intestine with commensal bacteria and is mediated by a pathway that requires TGF- β 1 and MyD88, but not TLR2.
American Psychological Association (APA)
Maria, Ann& English, Kathryn A.& Gorham, James D.. 2014. Appropriate Development of the Liver Treg Compartment Is Modulated by the Microbiota and Requires TGF-β and MyD88. Journal of Immunology Research،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1040820
Modern Language Association (MLA)
Maria, Ann…[et al.]. Appropriate Development of the Liver Treg Compartment Is Modulated by the Microbiota and Requires TGF-β and MyD88. Journal of Immunology Research No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-1040820
American Medical Association (AMA)
Maria, Ann& English, Kathryn A.& Gorham, James D.. Appropriate Development of the Liver Treg Compartment Is Modulated by the Microbiota and Requires TGF-β and MyD88. Journal of Immunology Research. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1040820
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1040820