Palladium Nanoparticles Induce Disturbances in Cell Cycle Entry and Progression of Peripheral Blood Mononuclear Cells: Paramount Role of Ions
Joint Authors
Bernardini, Giovanni
Petrarca, Claudia
Clemente, Emanuela
Di Giampaolo, Luca
Leopold, Kerstin
Schindl, Roland
Lotti, Lavinia V.
Mangifesta, Rocco
Sabbioni, Enrico
Niu, Qiao
Di Gioacchino, Mario
Mariani-Costantini, Renato
Source
Journal of Immunology Research
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-03
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
There is concern about the possible toxicity of palladium nanoparticles (Pd-NP), as they are released in the environment through many applications.
We previously studied the toxicity of Pd-NP at high concentrations; here we address the possible toxicity of Pd-NP at low, subtoxic doses.
In particular, we have exposed normal human PBMC entering into the first in vitro mitotic division to Pd-NP and to Pd(IV) ions to evaluate ROS generation and cell cycle progression.
We have measured a statistically significant increase of intracellular ROS in Pd(IV) exposed cells, but not in Pd-NP exposed cells.
TEM revealed accumulation of lipid droplets and autophagic and mitophagic vacuoles, which appeared more conspicuous in cells exposed to Pd(IV) ions than to Pd-NP.
Pd-NP were visible in the cytoplasm of Pd-NP exposed cells.
Pd-NP addition was associated with a significant increase of cells within the G0/G1-phase and a significant reduction in GS- and G2/M-phases.
Cells exposed to Pd(IV) ions showed a significant amplification of these cell cycle alterations.
These results suggest that ions, per se or released by NPs, are the true inducers of Pd toxicity.
It will be essential to verify whether the observed disturbance represents a temporary response or might result in permanent alterations.
American Psychological Association (APA)
Petrarca, Claudia& Clemente, Emanuela& Di Giampaolo, Luca& Mariani-Costantini, Renato& Leopold, Kerstin& Schindl, Roland…[et al.]. 2014. Palladium Nanoparticles Induce Disturbances in Cell Cycle Entry and Progression of Peripheral Blood Mononuclear Cells: Paramount Role of Ions. Journal of Immunology Research،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1040826
Modern Language Association (MLA)
Petrarca, Claudia…[et al.]. Palladium Nanoparticles Induce Disturbances in Cell Cycle Entry and Progression of Peripheral Blood Mononuclear Cells: Paramount Role of Ions. Journal of Immunology Research No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1040826
American Medical Association (AMA)
Petrarca, Claudia& Clemente, Emanuela& Di Giampaolo, Luca& Mariani-Costantini, Renato& Leopold, Kerstin& Schindl, Roland…[et al.]. Palladium Nanoparticles Induce Disturbances in Cell Cycle Entry and Progression of Peripheral Blood Mononuclear Cells: Paramount Role of Ions. Journal of Immunology Research. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1040826
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1040826