Identification of Osteosarcoma-Related Specific Proteins in Serum Samples Using Surface-Enhanced Laser DesorptionIonization-Time-of-Flight Mass Spectrometry
Joint Authors
Gu, Jianli
Li, Jitian
Huang, Manyu
Zhang, Zhiyong
Li, Dongsheng
Song, Guoying
Ding, Xingpo
Li, Wuyin
Source
Journal of Immunology Research
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-24
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Osteosarcoma (OS) is the most common malignant bone tumor.
To identify OS-related specific proteins for early diagnosis of OS, a novel approach, surface-enhanced laser desorption/ionization-time-of-flight mass spectrometry (SELDI-TOF-MS) to serum samples from 25 OS patients, 16 osteochondroma, and 26 age-matched normal human volunteers as controls, was performed.
Two proteins showed a significantly different expression in OS serum samples from control groups.
Proteomic profiles and external leave-one-out cross-validation analysis showed that the correct rate of allocation, the sensitivity, and the specificity of diagnosis were 100%.
These two proteins were further identified by searching the EPO-KB database, and one of the proteins identified as Serine rich region profile is involved in various cellular signaling cascades and tumor genesis.
The presence of these two proteins in OS patients but absence from premalignant and normal human controls implied that they can be potential biomarkers for early diagnosis of OS.
American Psychological Association (APA)
Gu, Jianli& Li, Jitian& Huang, Manyu& Zhang, Zhiyong& Li, Dongsheng& Song, Guoying…[et al.]. 2014. Identification of Osteosarcoma-Related Specific Proteins in Serum Samples Using Surface-Enhanced Laser DesorptionIonization-Time-of-Flight Mass Spectrometry. Journal of Immunology Research،Vol. 2014, no. 2014, pp.1-5.
https://search.emarefa.net/detail/BIM-1040934
Modern Language Association (MLA)
Gu, Jianli…[et al.]. Identification of Osteosarcoma-Related Specific Proteins in Serum Samples Using Surface-Enhanced Laser DesorptionIonization-Time-of-Flight Mass Spectrometry. Journal of Immunology Research No. 2014 (2014), pp.1-5.
https://search.emarefa.net/detail/BIM-1040934
American Medical Association (AMA)
Gu, Jianli& Li, Jitian& Huang, Manyu& Zhang, Zhiyong& Li, Dongsheng& Song, Guoying…[et al.]. Identification of Osteosarcoma-Related Specific Proteins in Serum Samples Using Surface-Enhanced Laser DesorptionIonization-Time-of-Flight Mass Spectrometry. Journal of Immunology Research. 2014. Vol. 2014, no. 2014, pp.1-5.
https://search.emarefa.net/detail/BIM-1040934
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1040934