Nuclear Pattern of CXCR4 Expression Is Associated with a Better Overall Survival in Patients with Gastric Cancer
Joint Authors
Nikkhoo, Bahram
Jalili, Ali
Fakhari, Shohreh
Sheikhesmaili, Farshad
Fathi, Fardin
Rooshani, Daem
Hoseinpour Feizi, Mohammad Ali
Nikzaban, Mehrnoush
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-02-10
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Introduction.
Previous studies have shown that stromal-derived factor-1 (CXCL12) and its receptor, CXCR4, play a crucial role in metastasis of various tumors.
Similarly, it has been cleared that CXCR4 is expressed on the cell surface of gastric cancers.
However, nuclear expression of CXCR4 and its clinical importance have not been yet studied.
Materials and Methods.
Herein, we studied the expression of CXCR4 in gastric samples from patients with gastric adenocarcinoma as well as human gastric carcinoma cell line, AGS, by employing RT-PCR, immunohistochemistry, and flow cytometry techniques.
Results.
RT-PCR data showed that CXCR4 is highly expressed on AGS cells.
This was confirmed by IHC and FACS as CXCR4 was detected on cell membrane, in cytoplasm, and in nucleus of AGS cells.
Moreover, we found that both cytoplasmic and nuclear CXCR4 are strongly expressed in primary gastric cancer and the cytoplasmic pattern of CXCR4 tends to be associated with a shorter overall survival than nuclear staining.
In conclusion, we present evidence for the first time that both cytoplasmic and nuclear expression of CXCR4 are detectable in gastric cancer tissues.
However, the role of both cytoplasmic and nuclear CXCR4 needs to be further elucidated.
American Psychological Association (APA)
Nikkhoo, Bahram& Jalili, Ali& Fakhari, Shohreh& Sheikhesmaili, Farshad& Fathi, Fardin& Rooshani, Daem…[et al.]. 2014. Nuclear Pattern of CXCR4 Expression Is Associated with a Better Overall Survival in Patients with Gastric Cancer. Journal of Oncology،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1042285
Modern Language Association (MLA)
Nikkhoo, Bahram…[et al.]. Nuclear Pattern of CXCR4 Expression Is Associated with a Better Overall Survival in Patients with Gastric Cancer. Journal of Oncology No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-1042285
American Medical Association (AMA)
Nikkhoo, Bahram& Jalili, Ali& Fakhari, Shohreh& Sheikhesmaili, Farshad& Fathi, Fardin& Rooshani, Daem…[et al.]. Nuclear Pattern of CXCR4 Expression Is Associated with a Better Overall Survival in Patients with Gastric Cancer. Journal of Oncology. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-1042285
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1042285