Liquid Chromatography-Tandem Mass Spectrometric Assay for Determination of Stavudine in Human Plasma
Author
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-17
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
A LC-MS/MS method for determination of stavudine in human plasma was established and validated, and it was applied to the pharmaceutical formulations bioequivalence study.
0.5 mL plasma sample was extracted by liquid-liquid extraction.
Stavudine was detected by a LC-MS/MS system.
The pharmacokinetic parameters of stavudine in different formulations were calculated by noncompartment model statistics.
The method was linear over the concentration ranges 5.00–1000 ng/mL in plasma.
The intra- and interassay relative standard deviation (RSD) was <10%.
The average accuracies for the assay at three concentrations (5.00, 80.0, and 900 ng/mL) were from 100.2% to 102.5%.
Pharmacokinetic parameters of stavudine reference formulation were obtained as follows: Tmax was 0.6±0.2 h, Cmax was 480.7±150.9 g/L, t1/2 was 1.7±0.4 h, and AUC0-t was 872.8±227.8 g·h/L, and pharmacokinetic parameters of stavudine test formulation were obtained as follows: Tmax was 0.5±0.2 h, Cmax was 537.5±178.5 g/L, t1/2 was 1.7±0.3 h, and AUC0-t was (914.1±284.5) g·h/L.
Calculated with AUC0-t, the bioavailability of two formulations was 105.0%.
American Psychological Association (APA)
Jin, Fengdan. 2014. Liquid Chromatography-Tandem Mass Spectrometric Assay for Determination of Stavudine in Human Plasma. Journal of Spectroscopy،Vol. 2014, no. 2014, pp.1-5.
https://search.emarefa.net/detail/BIM-1043103
Modern Language Association (MLA)
Jin, Fengdan. Liquid Chromatography-Tandem Mass Spectrometric Assay for Determination of Stavudine in Human Plasma. Journal of Spectroscopy No. 2014 (2014), pp.1-5.
https://search.emarefa.net/detail/BIM-1043103
American Medical Association (AMA)
Jin, Fengdan. Liquid Chromatography-Tandem Mass Spectrometric Assay for Determination of Stavudine in Human Plasma. Journal of Spectroscopy. 2014. Vol. 2014, no. 2014, pp.1-5.
https://search.emarefa.net/detail/BIM-1043103
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1043103