Gene Expression Profile in Delay Graft Function: Inflammatory Markers Are Associated with Recipient and Donor Risk Factors
Joint Authors
Guerrieri, Diego
Re, Luis
Petroni, Jorgelina
Ambrosi, Nella
Pilotti, Roxana E.
Chuluyan, H. Eduardo
Casadei, Domingo
Incardona, Claudio
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-18
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Background.
Delayed graft function (DGF) remains an important problem after kidney transplantation and reduced long-term graft survival of the transplanted organ.
The aim of the present study was to determine if the development of DGF was associated with a specific pattern of inflammatory gene expression in expanded criteria of deceased donor kidney transplantation.
Also, we explored the presence of correlations between DGF risk factors and the profile that was found.
Methods.
Seven days after kidney transplant, a cDNA microarray was performed on biopsies of graft from patients with and without DGF.
Data was confirmed by real-time PCR.
Correlations were performed between inflammatory gene expression and clinical risk factors.
Results.
From a total of 84 genes analyzed, 58 genes were upregulated while only 1 gene was downregulated in patients with DGF compared with no DGF (P=0.01).
The most relevant genes fold changes observed was IFNA1, IL-10, IL-1F7, IL-1R1, HMOX-1, and TGF-β.
The results were confirmed for IFNA1, IL-1R1, HMOX-1 and TGF-β.
A correlation was observed between TGF-β, donor age, and preablation creatinine, but not body mass index (BMI).
Also, TGF-β showed an association with recipient age, while IFNA1 correlated with recipient BMI.
Furthermore, TGF-β, IFNA1 and HMOX-1 correlated with several posttransplant kidney function markers, such as diuresis, ultrasound Doppler, and glycemia.
Conclusions.
Overall, the present study shows that DGF is associated with inflammatory markers, which are correlated with donor and recipient DGF risk factors.
American Psychological Association (APA)
Guerrieri, Diego& Re, Luis& Petroni, Jorgelina& Ambrosi, Nella& Pilotti, Roxana E.& Chuluyan, H. Eduardo…[et al.]. 2014. Gene Expression Profile in Delay Graft Function: Inflammatory Markers Are Associated with Recipient and Donor Risk Factors. Mediators of Inflammation،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1043352
Modern Language Association (MLA)
Guerrieri, Diego…[et al.]. Gene Expression Profile in Delay Graft Function: Inflammatory Markers Are Associated with Recipient and Donor Risk Factors. Mediators of Inflammation No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1043352
American Medical Association (AMA)
Guerrieri, Diego& Re, Luis& Petroni, Jorgelina& Ambrosi, Nella& Pilotti, Roxana E.& Chuluyan, H. Eduardo…[et al.]. Gene Expression Profile in Delay Graft Function: Inflammatory Markers Are Associated with Recipient and Donor Risk Factors. Mediators of Inflammation. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1043352
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1043352