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Krüppel-Like Factor 5 Mediates Proinflammatory Cytokine Expression in Lipopolysaccharide-Induced Acute Lung Injury through Upregulation of Nuclear Factor- κB Phosphorylation In Vitro and In Vivo
Joint Authors
Liu, Po-Len
Wang, Hui-Min
Chen, Hsiu-Lin
Chong, Inn-Wen
Lee, Yi-Chen
Tsai, Jong-Rung
Yuan, Shyng-Shiou F.
Liu, Wei-Lun
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-08-10
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Acute lung injury (ALI) is associated with an inflammation-mediated process, and the transcription factor, Krüppel-like factor 5 (KLF5), might play a crucial role in inflammatory lung disease.
In this study, we evaluated KLF5, reactive oxygen species (ROS), and inflammatory responses in a lipopolysaccharide- (LPS-) induced ALI model to elucidate the role of KLF5 in ALI.
Our data indicated that LPS upregulates proinflammatory cytokine expression in human bronchial epithelial cells in a dose-dependent manner.
We observed upregulated KLF5 protein expression in human bronchial epithelial cells exposed to LPS, with peak expression 1 h after LPS treatment, and subsequent upregulation of p65 protein expression and p65 phosphorylation at Ser276.
These results indicate that KLF5 mediates proinflammatory cytokine expression by upregulating nuclear factor-kappaB (NF-κB) phosphorylation at p65 in response to LPS.
LPS treatment also increased ROS production and simultaneously upregulated KLF5 expression and NF-κB translocation.
N-acetylcysteine significantly reduced ROS levels and KLF5 and NF-κB translocation in nuclear extracts.
Therefore, N-acetylcysteine pretreatment before LPS exposure reduces ROS, downregulates KLF5 expression, and subsequently reduces inflammatory responses by scavenging ROS.
Overall, our study results indicate that KLF5 mediates proinflammatory cytokine expression through upregulation of NF-κB phosphorylation at p65 in LPS-induced ALI.
American Psychological Association (APA)
Chen, Hsiu-Lin& Chong, Inn-Wen& Lee, Yi-Chen& Tsai, Jong-Rung& Yuan, Shyng-Shiou F.& Wang, Hui-Min…[et al.]. 2014. Krüppel-Like Factor 5 Mediates Proinflammatory Cytokine Expression in Lipopolysaccharide-Induced Acute Lung Injury through Upregulation of Nuclear Factor- κB Phosphorylation In Vitro and In Vivo. Mediators of Inflammation،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1043418
Modern Language Association (MLA)
Chen, Hsiu-Lin…[et al.]. Krüppel-Like Factor 5 Mediates Proinflammatory Cytokine Expression in Lipopolysaccharide-Induced Acute Lung Injury through Upregulation of Nuclear Factor- κB Phosphorylation In Vitro and In Vivo. Mediators of Inflammation No. 2014 (2014), pp.1-12.
https://search.emarefa.net/detail/BIM-1043418
American Medical Association (AMA)
Chen, Hsiu-Lin& Chong, Inn-Wen& Lee, Yi-Chen& Tsai, Jong-Rung& Yuan, Shyng-Shiou F.& Wang, Hui-Min…[et al.]. Krüppel-Like Factor 5 Mediates Proinflammatory Cytokine Expression in Lipopolysaccharide-Induced Acute Lung Injury through Upregulation of Nuclear Factor- κB Phosphorylation In Vitro and In Vivo. Mediators of Inflammation. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1043418
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1043418