Hesperidin Inhibits Inflammatory Response Induced by Aeromonas hydrophila Infection and Alters CD4 +CD8 + T Cell Ratio

Abstract EN

Background.

Aeromonas hydrophila is an opportunistic bacterial pathogen that is associated with a number of human diseases.

Hesperidin (HES) has been reported to exert antioxidant and anti-inflammatory activities.

Objectives.

The aim of this study was to investigate the potential effect of HES treatment on inflammatory response induced by A.

hydrophila infection in murine.

Methods.

A.

hydrophila-infected mice were treated with HES at 250 mg/kg b.wt./week for 4 consecutive weeks.

Phagocytosis, reactive oxygen species production, CD4+/CD8+ T cell ratio, and CD14 expression on intestinal infiltrating monocytes were evaluated.

The expression of E-selectin and intercellular adhesion molecule 1 on stimulated HUVECs and RAW macrophage was evaluated.

Results.

Percentage of CD4+ T cells in the intestinal tissues of infected treated mice was highly significantly increased; however, phagocytic index, ROS production, CD8+ T cells percentage, and CD14 expression on monocytes were significantly reduced.

On the other hand, HES significantly inhibited A-LPS- and A-ECP-induced E-selectin and ICAM-1 expression on HUVECs and ICAM-1 expression on RAW macrophage.

Conclusion.

Present data indicated that HES has a potential role in the suppression of inflammatory response induced by A.

hydrophila toxins through downmodulation of ROS production and CD14 and adhesion molecules expression, as well as increase of CD4+/CD8+ cell ratio.