Lycopene Modulates THP1 and Caco2 Cells Inflammatory State through Transcriptional and Nontranscriptional Processes
Joint Authors
Makon-Sébastien, Njock
Francis, Fouchier
Eric, Seree
Henri, Villard Pierre
François, Landrier Jean
Laurent, Pechere
Yves, Barra
Serge, Champion
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-07
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
We revisited the action of a carotenoid, the lycopene, on the expression of proinflammatory genes, reactive oxygen species (ROS) production, and metalloprotease (MMP9) activity.
THP1 and Caco2 cell lines were used as in vitro models for the two main cell types found in intestine tissue, that is, monocytes and epithelial cells.
Proinflammatory condition was induced using either phorbol ester acetate (PMA), lipopolysaccharide (LPS) or tumor necrosis factor (TNF).
In THP1 cells, short term pretreatment (2 h) with a low concentration (2 μM) of lycopene reinforce proinflammatory gene expression.
The extent of the effect of lycopene is dependent on the proinflammtory stimulus (PMA, LPS or TNF) used.
Lycopene enhanced MMP9 secretion via a c-AMP-dependent process, and reduced ROS production at higher concentrations than 2 μM.
Cell culture media, conditioned by PMA-treated monocytes and then transferred on CaCo-2 epithelial cells, induced a proinflammatory state in these cells.
The extent of this inflammatory effect was reduced when cells has been pretreated (12 h) with lycopene.
At low concentration (2 μM or less), lycopene appeared to promote an inflammatory state not correlated with ROS modulation.
At higher concentration (5 μM–20 μM), an anti-inflammatory effect takes place as a decrease of ROS production was detected.
So, both concentration and time have to be considered in order to define the exact issue of the effect of carotenoids present in meals.
American Psychological Association (APA)
Makon-Sébastien, Njock& Francis, Fouchier& Eric, Seree& Henri, Villard Pierre& François, Landrier Jean& Laurent, Pechere…[et al.]. 2014. Lycopene Modulates THP1 and Caco2 Cells Inflammatory State through Transcriptional and Nontranscriptional Processes. Mediators of Inflammation،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1043622
Modern Language Association (MLA)
Makon-Sébastien, Njock…[et al.]. Lycopene Modulates THP1 and Caco2 Cells Inflammatory State through Transcriptional and Nontranscriptional Processes. Mediators of Inflammation No. 2014 (2014), pp.1-12.
https://search.emarefa.net/detail/BIM-1043622
American Medical Association (AMA)
Makon-Sébastien, Njock& Francis, Fouchier& Eric, Seree& Henri, Villard Pierre& François, Landrier Jean& Laurent, Pechere…[et al.]. Lycopene Modulates THP1 and Caco2 Cells Inflammatory State through Transcriptional and Nontranscriptional Processes. Mediators of Inflammation. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1043622
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1043622