Elevated OPN, IP-10, and Neutrophilia in Loop-Mediated Isothermal Amplification Confirmed Tuberculosis Patients
Joint Authors
Suzuki, Yasuhiko
Telan, Elizabeth Freda O.
Shiratori, Beata
Nakajima, Chie
Chagan-Yasutan, Haorile
Niki, Toshiro
Ashino, Yugo
Hattori, Toshio
Leano, Prisca Susan A.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-10-14
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Tuberculosis (TB) is the second most common cause of death from infectious diseases and results in high socioeconomic losses to many countries.
Proper diagnosis is the first step in TB eradication.
To develop a rapid, simple, and accurate diagnostic TB test and to characterize the prevalence of Mycobacterium tuberculosis (MTB) genotypes and immune profiles of TB patients, a total of 37 TB patients and 30 healthy control (HC) from Metro Manila were enrolled.
Loop-mediated isothermal amplification (LAMP) reliably detected MTB infection.
Manila genotype was identified by spoligotyping method in all TB patients.
Osteopontin (OPN), interferon- γ -induced protein 10 kDa (IP-10), and neutrophil counts were found to reflect the acute stage of MTB infection.
The sensitivity and specificity were 94.6% and 93.3%, respectively, for both OPN and IP-10, and they were 83.8% and 78.6%, respectively, for neutrophils.
The combination of OPN, IP-10, neutrophil count, IL-6, IL-8, TNF- α , MCP-1, platelets, galectin-9, and leukocyte count correctly identifies all the HC and 96.3% of TB patients.
LAMP method may serve as a rapid, supportive method in addition to time-consuming culture methods.
OPN, IP-10, and neutrophil counts are useful in detecting MTB infection and may have utility in monitoring the course of the disease.
American Psychological Association (APA)
Shiratori, Beata& Leano, Prisca Susan A.& Nakajima, Chie& Chagan-Yasutan, Haorile& Niki, Toshiro& Ashino, Yugo…[et al.]. 2014. Elevated OPN, IP-10, and Neutrophilia in Loop-Mediated Isothermal Amplification Confirmed Tuberculosis Patients. Mediators of Inflammation،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1043627
Modern Language Association (MLA)
Shiratori, Beata…[et al.]. Elevated OPN, IP-10, and Neutrophilia in Loop-Mediated Isothermal Amplification Confirmed Tuberculosis Patients. Mediators of Inflammation No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-1043627
American Medical Association (AMA)
Shiratori, Beata& Leano, Prisca Susan A.& Nakajima, Chie& Chagan-Yasutan, Haorile& Niki, Toshiro& Ashino, Yugo…[et al.]. Elevated OPN, IP-10, and Neutrophilia in Loop-Mediated Isothermal Amplification Confirmed Tuberculosis Patients. Mediators of Inflammation. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-1043627
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1043627