Involvement of Different CD4+ T Cell Subsets Producing Granzyme B in the Immune Response to Leishmania major Antigens
Joint Authors
Naouar, Ikbel
Boussoffara, Thouraya
Ben Ahmed, Melika
Belhaj Hmida, Nabil
Gharbi, Adel
Gritli, Sami
Ben Salah, Afif
Louzir, Hechmi
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-01
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The nature of effector cells and the potential immunogenicity of Leishmania major excreted/secreted proteins (LmES) were evaluated using peripheral blood mononuclear cells (PBMCs) from healed zoonotic cutaneous leishmaniasis individuals (HZCL) and healthy controls (HC).
First, we found that PBMCs from HZCL individuals proliferate and produce high levels of IFN-γ and granzyme B (GrB), used as a marker of activated cytotoxic T cells, in response to the parasite antigens.
IFN-γ is produced by CD4+ T cells, but unexpectedly GrB is also produced by CD4+ T cells in response to stimulation with LmES, which were found to be as effective as soluble Leishmania antigens to induce proliferation and cytokine production by PBMCs from immune individuals.
To address the question of regulatory T cell (Tregs) involvement, the frequency of circulating Tregs was assessed and found to be higher in HZCL individuals compared to that of HC.
Furthermore, both CD4+CD25+ and CD4+CD25− T cells, purified from HZCL individuals, produced IFN-γ and GrB when stimulated with LmES.
Additional experiments showed that CD 4 + CD 25 + CD 127 dim/ - Tregs were involved in GrB production.
Collectively, our data indicate that LmES are immunogenic in humans and emphasize the involvement of CD4+ T cells including activated and regulatory T cells in the immune response against parasite antigens.
American Psychological Association (APA)
Naouar, Ikbel& Boussoffara, Thouraya& Ben Ahmed, Melika& Belhaj Hmida, Nabil& Gharbi, Adel& Gritli, Sami…[et al.]. 2014. Involvement of Different CD4+ T Cell Subsets Producing Granzyme B in the Immune Response to Leishmania major Antigens. Mediators of Inflammation،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1043695
Modern Language Association (MLA)
Naouar, Ikbel…[et al.]. Involvement of Different CD4+ T Cell Subsets Producing Granzyme B in the Immune Response to Leishmania major Antigens. Mediators of Inflammation No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1043695
American Medical Association (AMA)
Naouar, Ikbel& Boussoffara, Thouraya& Ben Ahmed, Melika& Belhaj Hmida, Nabil& Gharbi, Adel& Gritli, Sami…[et al.]. Involvement of Different CD4+ T Cell Subsets Producing Granzyme B in the Immune Response to Leishmania major Antigens. Mediators of Inflammation. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1043695
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1043695