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Chronic Ethanol Feeding Modulates Inflammatory Mediators, Activation of Nuclear Factor-κB, and Responsiveness to Endotoxin in Murine Kupffer Cells and Circulating Leukocytes
Joint Authors
Marzi, Ingo
Lehnert, Mark
Maraslioglu, Miriam
Oppermann, Elsie
Blattner, Carolin
Weber, Roxane
Jobin, Christian
Schleucher, Elke
Henrich, Dirk
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-01-29
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
Chronic ethanol abuse is known to increase susceptibility to infections after injury, in part, by modification of macrophage function.
Several intracellular signalling mechanisms are involved in the initiation of inflammatory responses, including the nuclear factor-κB (NF-κB) pathway.
In this study, we investigated the systemic and hepatic effect of chronic ethanol feeding on in vivo activation of NF-κB in NF-κBEGFP reporter gene mice.
Specifically, the study focused on Kupffer cell proinflammatory cytokines IL-6 and TNF-α and activation of NF-κB after chronic ethanol feeding followed by in vitro stimulation with lipopolysaccharide (LPS).
We found that chronic ethanol upregulated NF-κB activation and increased hepatic and systemic proinflammatory cytokine levels.
Similarly, LPS-stimulated IL-1β release from whole blood was significantly enhanced in ethanol-fed mice.
However, LPS significantly increased IL-6 and TNF-α levels.
These results demonstrate that chronic ethanol feeding can improve the responsiveness of macrophage LPS-stimulated IL-6 and TNF-α production and indicate that this effect may result from ethanol-induced alterations in intracellular signalling through NF-κB.
Furthermore, LPS and TNF-α stimulated the gene expression of different inflammatory mediators, in part, in a NF-κB-dependent manner.
American Psychological Association (APA)
Maraslioglu, Miriam& Oppermann, Elsie& Blattner, Carolin& Weber, Roxane& Henrich, Dirk& Jobin, Christian…[et al.]. 2014. Chronic Ethanol Feeding Modulates Inflammatory Mediators, Activation of Nuclear Factor-κB, and Responsiveness to Endotoxin in Murine Kupffer Cells and Circulating Leukocytes. Mediators of Inflammation،Vol. 2014, no. 2014, pp.1-16.
https://search.emarefa.net/detail/BIM-1043823
Modern Language Association (MLA)
Maraslioglu, Miriam…[et al.]. Chronic Ethanol Feeding Modulates Inflammatory Mediators, Activation of Nuclear Factor-κB, and Responsiveness to Endotoxin in Murine Kupffer Cells and Circulating Leukocytes. Mediators of Inflammation No. 2014 (2014), pp.1-16.
https://search.emarefa.net/detail/BIM-1043823
American Medical Association (AMA)
Maraslioglu, Miriam& Oppermann, Elsie& Blattner, Carolin& Weber, Roxane& Henrich, Dirk& Jobin, Christian…[et al.]. Chronic Ethanol Feeding Modulates Inflammatory Mediators, Activation of Nuclear Factor-κB, and Responsiveness to Endotoxin in Murine Kupffer Cells and Circulating Leukocytes. Mediators of Inflammation. 2014. Vol. 2014, no. 2014, pp.1-16.
https://search.emarefa.net/detail/BIM-1043823
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1043823