Dual Effect of Exogenous Nitric Oxide on Neuronal Excitability in Rat Substantia Gelatinosa Neurons
Joint Authors
Park, A-Reum
Lee, Hae In
Semjid, Dejidnorov
Kim, Do Kyung
Chun, Sang Woo
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-01-08
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Nitric oxide (NO) is an important signaling molecule involved in nociceptive transmission.
It can induce analgesic and hyperalgesic effects in the central nervous system.
In this study, patch-clamp recording was used to investigate the effect of NO on neuronal excitability in substantia gelatinosa (SG) neurons of the spinal cord.
Different concentrations of sodium nitroprusside (SNP; NO donor) induced a dual effect on the excitability of neuronal membrane: 1 mM of SNP evoked membrane hyperpolarization and an outward current, whereas 10 µM induced depolarization of the membrane and an inward current.
These effects were prevented by hemoglobin and 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide potassium salt (c-PTIO) (NO scavengers), phenyl N-tert-butylnitrone (PBN; nonspecific reactive oxygen species scavenger), and through inhibition of soluble guanylyl cyclase (sGC).
Pretreatment with n-ethylmaleimide (NEM; thiol-alkylating agent) also decreased effects of both 1 mM and 10 µM SNP, suggesting that these responses were mediated by direct S-nitrosylation.
Charybdotoxin (CTX) and tetraethylammonium (TEA) (large-conductance Ca2+-activated K+ channel blockers) and glybenclamide (ATP-sensitive K+ channel blocker) decreased SNP-induced hyperpolarization.
La3+ (nonspecific cation channel blocker), but not Cs+ (hyperpolarization-activated K+ channel blocker), blocked SNP-induced membrane depolarization.
In conclusion, NO dually affects neuronal excitability in a concentration-dependent manner via modification of various K+ channels.
American Psychological Association (APA)
Park, A-Reum& Lee, Hae In& Semjid, Dejidnorov& Kim, Do Kyung& Chun, Sang Woo. 2014. Dual Effect of Exogenous Nitric Oxide on Neuronal Excitability in Rat Substantia Gelatinosa Neurons. Neural Plasticity،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1046729
Modern Language Association (MLA)
Park, A-Reum…[et al.]. Dual Effect of Exogenous Nitric Oxide on Neuronal Excitability in Rat Substantia Gelatinosa Neurons. Neural Plasticity No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-1046729
American Medical Association (AMA)
Park, A-Reum& Lee, Hae In& Semjid, Dejidnorov& Kim, Do Kyung& Chun, Sang Woo. Dual Effect of Exogenous Nitric Oxide on Neuronal Excitability in Rat Substantia Gelatinosa Neurons. Neural Plasticity. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1046729
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1046729