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Vitamin A Supplementation Alleviates Extrahepatic Cholestasis Liver Injury through Nrf2 Activation
Joint Authors
Wang, Guiyang
Xiu, Peng
Li, Fu
Xin, Cheng
Li, Kewei
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-09
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Aim.
To investigate the role of vitamin A in liver damage induced by bile duct ligation (BDL) in rats.
Methods.
Thirty male Wistar rats were randomly divided into three groups: SHAM group, BDL group, and BDL + VitA group .
The concentrations of retinol and retinyl palmitate in the liver were analyzed using HPLC, and liver function was evaluated by the level of TBIL, ALT, AST, and ALP in serum.
Hepatic oxidative status was estimated by measuring T-SOD, CAT, GSH, MDA, and AOPP.
Nrf2 expression was assessed using immunohistochemistry and western blotting, and EMSA was performed to determine Nrf2 DNA-binding activity.
The expression of the downstream factors such as Ho1 and Nqo1 was also examined using immunohistochemistry and western blotting assays.
Results.
Vitamin A treatment restored levels of retinoids in liver, improved liver function, alleviated oxidative stress, and facilitated the translocation of Nrf2 to the nucleus in the experimental obstructive jaundice.
Vitamin A was also found to increase the expression of Nrf2 downstream proteins such as Ho1 and Nqo1.
Conclusion.
Vitamin A was here found to ameliorate cholestatic liver injury.
This effect may be related to the activation of Nrf2/ARE pathway in bile duct ligation rats.
American Psychological Association (APA)
Wang, Guiyang& Xiu, Peng& Li, Fu& Xin, Cheng& Li, Kewei. 2014. Vitamin A Supplementation Alleviates Extrahepatic Cholestasis Liver Injury through Nrf2 Activation. Oxidative Medicine and Cellular Longevity،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1046985
Modern Language Association (MLA)
Wang, Guiyang…[et al.]. Vitamin A Supplementation Alleviates Extrahepatic Cholestasis Liver Injury through Nrf2 Activation. Oxidative Medicine and Cellular Longevity No. 2014 (Dec. 2014), pp.1-10.
https://search.emarefa.net/detail/BIM-1046985
American Medical Association (AMA)
Wang, Guiyang& Xiu, Peng& Li, Fu& Xin, Cheng& Li, Kewei. Vitamin A Supplementation Alleviates Extrahepatic Cholestasis Liver Injury through Nrf2 Activation. Oxidative Medicine and Cellular Longevity. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-1046985
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1046985