RCAN1 Regulates Mitochondrial Function and Increases Susceptibility to Oxidative Stress in Mammalian Cells
Joint Authors
Peiris, Heshan
Dubach, Daphne
Jessup, Claire F.
Unterweger, Petra
Raghupathi, Ravinarayan
Muyderman, Hakan
Zanin, Mark P.
Mackenzie, Kimberly
Pritchard, Melanie A.
Keating, Damien J.
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-09
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Mitochondria are the primary site of cellular energy generation and reactive oxygen species (ROS) accumulation.
Elevated ROS levels are detrimental to normal cell function and have been linked to the pathogenesis of neurodegenerative disorders such as Down's syndrome (DS) and Alzheimer’s disease (AD).
RCAN1 is abundantly expressed in the brain and overexpressed in brain of DS and AD patients.
Data from nonmammalian species indicates that increased RCAN1 expression results in altered mitochondrial function and that RCAN1 may itself regulate neuronal ROS production.
In this study, we have utilized mice overexpressing RCAN1 R C A N 1 o x and demonstrate an increased susceptibility of neurons from these mice to oxidative stress.
Mitochondria from these mice are more numerous and smaller, indicative of mitochondrial dysfunction, and mitochondrial membrane potential is altered under conditions of oxidative stress.
We also generated a PC12 cell line overexpressing RCAN1 P C 12 R C A N 1 .
Similar to R C A N 1 o x neurons, P C 12 R C A N 1 cells have an increased susceptibility to oxidative stress and produce more mitochondrial ROS.
This study demonstrates that increasing RCAN1 expression alters mitochondrial function and increases the susceptibility of neurons to oxidative stress in mammalian cells.
These findings further contribute to our understanding of RCAN1 and its potential role in the pathogenesis of neurodegenerative disorders such as AD and DS.
American Psychological Association (APA)
Peiris, Heshan& Dubach, Daphne& Jessup, Claire F.& Unterweger, Petra& Raghupathi, Ravinarayan& Muyderman, Hakan…[et al.]. 2014. RCAN1 Regulates Mitochondrial Function and Increases Susceptibility to Oxidative Stress in Mammalian Cells. Oxidative Medicine and Cellular Longevity،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1047051
Modern Language Association (MLA)
Peiris, Heshan…[et al.]. RCAN1 Regulates Mitochondrial Function and Increases Susceptibility to Oxidative Stress in Mammalian Cells. Oxidative Medicine and Cellular Longevity No. 2014 (Dec. 2014), pp.1-12.
https://search.emarefa.net/detail/BIM-1047051
American Medical Association (AMA)
Peiris, Heshan& Dubach, Daphne& Jessup, Claire F.& Unterweger, Petra& Raghupathi, Ravinarayan& Muyderman, Hakan…[et al.]. RCAN1 Regulates Mitochondrial Function and Increases Susceptibility to Oxidative Stress in Mammalian Cells. Oxidative Medicine and Cellular Longevity. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1047051
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1047051