SIRT1 Functions as an Important Regulator of Estrogen-Mediated Cardiomyocyte Protection in Angiotensin II-Induced Heart Hypertrophy
Joint Authors
Ruan, Yang
Shen, Tao
Man, Yong
Li, Jian
Ding, Ling
Qin, Weiwei
Lin, Yajun
Xi, Chao
Lu, Yonggang
Dou, Lin
Zhu, Yuping
Cao, Yuan
Bian, Yunfei
Wang, Shu
Xiao, Chuanshi
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-12-29
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Background.
Sirtuin 1 (SIRT1) is a member of the sirtuin family, which could activate cell survival machinery and has been shown to be protective in regulation of heart function.
Here, we determined the mechanism by which SIRT1 regulates Angiotensin II- (AngII-) induced cardiac hypertrophy and injury in vivo and in vitro.
Methods.
We analyzed SIRT1 expression in the hearts of control and AngII-induced mouse hypertrophy.
Female C57BL/6 mice were ovariectomized and pretreated with 17β-estradiol to measure SIRT1 expression.
Protein synthesis, cardiomyocyte surface area analysis, qRT-PCR, TUNEL staining, and Western blot were performed on AngII-induced mouse heart hypertrophy samples and cultured neonatal rat ventricular myocytes (NRVMs) to investigate the function of SIRT1.
Results.
SIRT1 expression was slightly upregulated in AngII-induced mouse heart hypertrophy in vivo and in vitro, accompanied by elevated cardiomyocyte apoptosis.
SIRT1 overexpression relieves AngII-induced cardiomyocyte hypertrophy and apoptosis.
17β-Estradiol was able to protect cardiomyocytes from AngII-induced injury with a profound upregulation of SIRT1 and activation of AMPK.
Moreover, estrogen receptor inhibitor ICI 182,780 and SIRT1 inhibitor niacinamide could block SIRT1’s protective effect.
Conclusions.
These results indicate that SIRT1 functions as an important regulator of estrogen-mediated cardiomyocyte protection during AngII-induced heart hypertrophy and injury.
American Psychological Association (APA)
Shen, Tao& Ding, Ling& Ruan, Yang& Qin, Weiwei& Lin, Yajun& Xi, Chao…[et al.]. 2014. SIRT1 Functions as an Important Regulator of Estrogen-Mediated Cardiomyocyte Protection in Angiotensin II-Induced Heart Hypertrophy. Oxidative Medicine and Cellular Longevity،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1047094
Modern Language Association (MLA)
Shen, Tao…[et al.]. SIRT1 Functions as an Important Regulator of Estrogen-Mediated Cardiomyocyte Protection in Angiotensin II-Induced Heart Hypertrophy. Oxidative Medicine and Cellular Longevity No. 2014 (Dec. 2014), pp.1-11.
https://search.emarefa.net/detail/BIM-1047094
American Medical Association (AMA)
Shen, Tao& Ding, Ling& Ruan, Yang& Qin, Weiwei& Lin, Yajun& Xi, Chao…[et al.]. SIRT1 Functions as an Important Regulator of Estrogen-Mediated Cardiomyocyte Protection in Angiotensin II-Induced Heart Hypertrophy. Oxidative Medicine and Cellular Longevity. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1047094
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1047094