Association between Paraoxonases Gene Expression and Oxidative Stress in Hepatotoxicity Induced by CCl4
Joint Authors
al-Harbi, Mohamed M.
Sayed-Ahmed, Mohammed M.
Al-Rejaie, Salim S.
Alsurayea, Saad M.
Hafez, Mohamed M.
al-Harbi, Naif O.
al-Shabanah, Othman A.
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-11-16
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Objectives.
The purpose of the study is to evaluate the hepatoprotective effect of rutin in carbon tetrachloride- (CCl4-) induced liver injuries in rat model.
Methods.
Forty male Wistar albino rats were divided into four groups.
Group I was the control group and received dimethyl sulphoxide (DMSO) and olive oil.
Group II received rutin.
Groups III was treated with CCl4.
Group IV was administered rutin after 48 h of CCl4 treatment.
Liver enzymes level, lipid profile, lipid peroxidation, and hydrogen peroxide were measured.
The genes expression levels were monitored by real time RT-PCR and western blot techniques.
Results.
CCl4 group showed significant increase in alanine aminotransferase (ALT), aspartate aminotransferase (AST), thiobarbituric acid reactive substances (TBAR), hydrogen peroxide (H2O2), and lipid profile and a significant decrease in glutathione peroxidase (GPx), glutathione S transferase (GST), catalase (CAT), paraoxonase-1 (PON-1), paraoxonase-3 (PON-3), peroxisome proliferator activated receptor delta (PPAR-δ), and ATP-binding cassette transporter 1 (ABAC1) genes expression levels.
Interestingly, rutin supplementation completely reversed the biochemical and gene expression levels induced by CCl4 to control values.
Conclusion.
CCl4 administration causes aberration of genes expression levels in oxidative stress pathway resulting in DNA damage and hepatotoxicity.
Rutin causes hepatoprotective effect through enhancing the antioxidant genes.
American Psychological Association (APA)
Hafez, Mohamed M.& al-Shabanah, Othman A.& al-Harbi, Naif O.& al-Harbi, Mohamed M.& Al-Rejaie, Salim S.& Alsurayea, Saad M.…[et al.]. 2014. Association between Paraoxonases Gene Expression and Oxidative Stress in Hepatotoxicity Induced by CCl4. Oxidative Medicine and Cellular Longevity،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1047154
Modern Language Association (MLA)
Hafez, Mohamed M.…[et al.]. Association between Paraoxonases Gene Expression and Oxidative Stress in Hepatotoxicity Induced by CCl4. Oxidative Medicine and Cellular Longevity No. 2014 (Dec. 2014), pp.1-12.
https://search.emarefa.net/detail/BIM-1047154
American Medical Association (AMA)
Hafez, Mohamed M.& al-Shabanah, Othman A.& al-Harbi, Naif O.& al-Harbi, Mohamed M.& Al-Rejaie, Salim S.& Alsurayea, Saad M.…[et al.]. Association between Paraoxonases Gene Expression and Oxidative Stress in Hepatotoxicity Induced by CCl4. Oxidative Medicine and Cellular Longevity. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1047154
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1047154