Directing Parthenogenetic Stem Cells Differentiate into Adipocytes for Engineering Injectable Adipose Tissue
Joint Authors
Yang, Xinyuan
Liu, Wei
Yan, Xingrong
Cui, Jihong
Liu, Wenguang
Sun, Mei
Rao, Yang
Chen, Fulin
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-12-23
Country of Publication
Egypt
No. of Pages
16
Abstract EN
The selection of appropriate seed cells is crucial for adipose tissue engineering.
Here, we reported the stepwise induction of parthenogenetic embryonic stem cells (pESCs) to differentiate into adipogenic cells and its application in engineering injectable adipose tissue with Pluronic F-127.
pESCs had pluripotent differentiation capacity and could form teratomas that include the three primary germ layers.
Cells that migrated from the embryoid bodies (EBs) were selectively separated and expanded to obtain embryonic mesenchymal stem cells (eMSCs).
The eMSCs exhibited similar cell surface marker expression profiles with bone morrow mesenchymal stem cells (BMSCs) and had multipotent differentiation capacity.
Under the induction of dexamethasone, indomethacin, and insulin, eMSCs could differentiate into adipogenic cells with increased expression of adipose-specific genes and oil droplet depositions within the cytoplasm.
To evaluate their suitability as seed cells for adipose tissue engineering, the CM-Dil labelled adipogenic cells derived from eMSCs were seeded into Pluronic F-127 hydrogel and injected subcutaneously into nude mice.
Four weeks after injection, glistering and semitransparent constructs formed in the subcutaneous site.
Histological observations demonstrated that new adipose tissue was successfully fabricated in the specimen by the labelled cells.
The results of the current study indicated that pESCs have great potential in the fabrication of injectable adipose tissue.
American Psychological Association (APA)
Liu, Wei& Yang, Xinyuan& Yan, Xingrong& Cui, Jihong& Liu, Wenguang& Sun, Mei…[et al.]. 2014. Directing Parthenogenetic Stem Cells Differentiate into Adipocytes for Engineering Injectable Adipose Tissue. Stem Cells International،Vol. 2014, no. 2014, pp.1-16.
https://search.emarefa.net/detail/BIM-1047515
Modern Language Association (MLA)
Liu, Wei…[et al.]. Directing Parthenogenetic Stem Cells Differentiate into Adipocytes for Engineering Injectable Adipose Tissue. Stem Cells International No. 2014 (2014), pp.1-16.
https://search.emarefa.net/detail/BIM-1047515
American Medical Association (AMA)
Liu, Wei& Yang, Xinyuan& Yan, Xingrong& Cui, Jihong& Liu, Wenguang& Sun, Mei…[et al.]. Directing Parthenogenetic Stem Cells Differentiate into Adipocytes for Engineering Injectable Adipose Tissue. Stem Cells International. 2014. Vol. 2014, no. 2014, pp.1-16.
https://search.emarefa.net/detail/BIM-1047515
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1047515