Histopathological Findings in Immunohistological Staining of the Granulomatous Tissue Reaction Associated with Tuberculosis
Joint Authors
Bahadori, Moslem
Karimi, Shirin
Shamaei, Masoud
Pourabdollah, Mihan
Sadr, Makan
Karbasi, Mehrdad
Kiani, Arda
Source
Tuberculosis Research and Treatment
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-01-05
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Purpose.
The histological diagnosis of Mycobacterium tuberculosis (MTB) remains a diagnostic challenge despite different methods.
Immunohistochemistry (IHC) not only could confirm granulomatous tissue involvement but also can demonstrate MTB antigen immunolocalization.
This study tries to clarify the details of immunohistochemical staining for MTB with pAbBCG.
Materials/Methods.
Twenty-three confirmed TB granulomatous tissue samples were studied by Ziehl-Neelsen and immunohistochemistry (IHC) staining with pAbBCG.
Samples were selected from the archive of the Department of Pathology, National Research Institute of Tuberculosis and Lung Disease, Tehran, Iran.
Results.
IHC staining was positive in all samples, whereas Ziehl-Neelsen was positive in 9 cases out of 23 (39.1%).
Tissue types used were pleural tissue, lymph nodes, and lung tissue.
IHC showed positive coarse granular cytoplasmic and round, fragmented bacillary staining.
In this study, epithelioid cells clearly showed more positive staining at the periphery of the granuloma rather than the center of granuloma.
There is also positive staining in endothelial cells, fibroblasts, plasma cells, lymphocytes, and macrophages outside the granuloma.
Conclusion.
Considering the criteria of positive immunohistochemical staining of TB granulomatous reactions, this stain not only highlights the presence of mycobacterial antigens for tissue diagnosis, but also could morphologically localize its distribution in different cells.
American Psychological Association (APA)
Karimi, Shirin& Shamaei, Masoud& Pourabdollah, Mihan& Sadr, Makan& Karbasi, Mehrdad& Kiani, Arda…[et al.]. 2014. Histopathological Findings in Immunohistological Staining of the Granulomatous Tissue Reaction Associated with Tuberculosis. Tuberculosis Research and Treatment،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1048075
Modern Language Association (MLA)
Karimi, Shirin…[et al.]. Histopathological Findings in Immunohistological Staining of the Granulomatous Tissue Reaction Associated with Tuberculosis. Tuberculosis Research and Treatment No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-1048075
American Medical Association (AMA)
Karimi, Shirin& Shamaei, Masoud& Pourabdollah, Mihan& Sadr, Makan& Karbasi, Mehrdad& Kiani, Arda…[et al.]. Histopathological Findings in Immunohistological Staining of the Granulomatous Tissue Reaction Associated with Tuberculosis. Tuberculosis Research and Treatment. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-1048075
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1048075