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Diverse Effects of Lead Nitrate on the Proliferation, Differentiation, and Gene Expression of Stem Cells Isolated from a Dental Origin
Joint Authors
Abu Kasim, Noor Hayaty
Musa, Sabri
Govindasamy, Vijayendran
Abdullah, Mariam
Rahman, Fazliny Abd.
Gnanasegaran, Nareshwaran
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-01-28
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Lead (Pb2+) exposure continues to be a significant public health problem.
Therefore, it is vital to have a continuous epidemiological dataset for a better understanding of Pb2+ toxicity.
In the present study, we have exposed stem cells isolated from deciduous and permanent teeth, periodontal ligament, and bone marrow to five different types of Pb2+ concentrations (160, 80, 40, 20, and 10 µM) for 24 hours to identify the adverse effects of Pb2+ on the proliferation, differentiation, and gene expression on these cell lines.
We found that Pb2+ treatment altered the morphology and adhesion of the cells in a dose-dependent manner.
There were no significant changes in terms of cell surface phenotypes.
Cells exposed to Pb2+ continued to differentiate into chondrogenesis and adipogenesis, and a severe downregulation was observed in osteogenesis.
Gene expression studies revealed a constant expression of key markers associated with stemness (Oct 4, Rex 1) and DNA repair enzyme markers, but downregulation occurred with some ectoderm and endoderm markers, demonstrating an irregular and untimely differentiation trail.
Our study revealed for the first time that Pb2+ exposure not only affects the phenotypic characteristics but also induces significant alteration in the differentiation and gene expression in the cells.
American Psychological Association (APA)
Abdullah, Mariam& Rahman, Fazliny Abd.& Gnanasegaran, Nareshwaran& Govindasamy, Vijayendran& Abu Kasim, Noor Hayaty& Musa, Sabri. 2014. Diverse Effects of Lead Nitrate on the Proliferation, Differentiation, and Gene Expression of Stem Cells Isolated from a Dental Origin. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1048826
Modern Language Association (MLA)
Abdullah, Mariam…[et al.]. Diverse Effects of Lead Nitrate on the Proliferation, Differentiation, and Gene Expression of Stem Cells Isolated from a Dental Origin. The Scientific World Journal No. 2014 (2014), pp.1-12.
https://search.emarefa.net/detail/BIM-1048826
American Medical Association (AMA)
Abdullah, Mariam& Rahman, Fazliny Abd.& Gnanasegaran, Nareshwaran& Govindasamy, Vijayendran& Abu Kasim, Noor Hayaty& Musa, Sabri. Diverse Effects of Lead Nitrate on the Proliferation, Differentiation, and Gene Expression of Stem Cells Isolated from a Dental Origin. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1048826
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1048826