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A Method for the Simultaneous Determination of Chlorogenic Acid and Sesquiterpene Lactone Content in Industrial Chicory Root Foodstuffs
Joint Authors
Hilbert, Jean-Louis
Willeman, Honorine
Fertin, Anne
Voedts, Najia
Duhal, Nathalie
Goossens, Jean-François
Hance, Philippe
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-12-04
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
A method for the simultaneous determination of free chlorogenic acids (CGA) and sesquiterpene lactones (STL) in chicory root and its dried (flour) and roasted (grain) forms is described.
The method uses one extraction and one analysis for all chicory root products.
Various solvents with low to high polarity, such as methanol, chloroform, or n-hexane, were tested alone, in combination in different proportions or with acidified or neutral aqueous solvent.
The water/chloroform/methanol (30/30/40, v/v/v) mixture generated the best extraction yield, 21% higher than alcohol mixtures.
The profiling of CGA and STL content was performed through a conventional HPLC-DAD method using a PFP core shell column in a fast single run.
Good retention time and area repeatability (RDD mean % 0.46 and 5.6, resp.) and linearity ( R 2 ≥ 0.96 ) were obtained.
The STL and chlorogenic acids levels determined were 254.7 and 100.2 μ g/g of dry matter in the root, 792.5 and 1,547 μ g/g in flour, and 160.4 and 822.5 μ g/g in the roasted grains, respectively.
With an average recovery of 106% and precision of 90%, this method is rapid, reproducible, and straightforward way to quantify the chlorogenic acids and STL in chicory raw material and end products.
American Psychological Association (APA)
Willeman, Honorine& Hance, Philippe& Fertin, Anne& Voedts, Najia& Duhal, Nathalie& Goossens, Jean-François…[et al.]. 2014. A Method for the Simultaneous Determination of Chlorogenic Acid and Sesquiterpene Lactone Content in Industrial Chicory Root Foodstuffs. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1050198
Modern Language Association (MLA)
Willeman, Honorine…[et al.]. A Method for the Simultaneous Determination of Chlorogenic Acid and Sesquiterpene Lactone Content in Industrial Chicory Root Foodstuffs. The Scientific World Journal No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-1050198
American Medical Association (AMA)
Willeman, Honorine& Hance, Philippe& Fertin, Anne& Voedts, Najia& Duhal, Nathalie& Goossens, Jean-François…[et al.]. A Method for the Simultaneous Determination of Chlorogenic Acid and Sesquiterpene Lactone Content in Industrial Chicory Root Foodstuffs. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-1050198
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1050198