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Protective Effects of Trehalose on the Corneal Epithelial Cells
Joint Authors
Aragona, Pasquale
Colosi, Pietro
Rania, Laura
Colosi, Francesca
Pisani, Antonina
Puzzolo, Domenico
Micali, Antonio
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-18
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Purpose.
Aim of the present work was to evaluate the effects of the trehalose on the corneal epithelium undergoing alcohol delamination.
Methods.
Twelve patients undergoing laser subepithelial keratomileusis (LASEK) were consecutively included in the study.
The right eyes were pretreated with 3% trehalose eye drops, whilst left eyes were used as control.
Epithelial specimens were processed for cells vitality assessment, apoptosis, and light and transmission electron microscopy; a morphometric analysis was performed in both groups.
Results.
In both trehalose-untreated eyes (TUE) and trehalose-treated eyes (TTE), the percentage of vital cells was similar and no apoptotic cells were observed.
In TUE, the corneal epithelium showed superficial cells with reduced microfolds, wing cells with vesicles and dilated intercellular spaces, and dark basal cells with vesicles and wide clefts.
In TTE, superficial and wing cells were better preserved, and basal cells were generally clear with intracytoplasmatic vesicles.
The morphometric analysis showed statistically significant differences between the two groups: the TTE epithelial height was higher, the basal cells showed larger area and clearer cytoplasm.
The distribution of desmosomes and hemidesmosomes was significantly different between the groups.
Conclusions.
Trehalose administration better preserved morphological and morphometric features of alcohol-treated corneal epithelium, when compared to controls.
American Psychological Association (APA)
Aragona, Pasquale& Colosi, Pietro& Rania, Laura& Colosi, Francesca& Pisani, Antonina& Puzzolo, Domenico…[et al.]. 2014. Protective Effects of Trehalose on the Corneal Epithelial Cells. The Scientific World Journal،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1050741
Modern Language Association (MLA)
Aragona, Pasquale…[et al.]. Protective Effects of Trehalose on the Corneal Epithelial Cells. The Scientific World Journal No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-1050741
American Medical Association (AMA)
Aragona, Pasquale& Colosi, Pietro& Rania, Laura& Colosi, Francesca& Pisani, Antonina& Puzzolo, Domenico…[et al.]. Protective Effects of Trehalose on the Corneal Epithelial Cells. The Scientific World Journal. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-1050741
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1050741