Arsenic Primes Human Bone Marrow CD34+ Cells for Erythroid Differentiation
Joint Authors
Zhang, Yuanyuan
Wang, Shasha
Chen, Chunyan
Wu, Xiao
Zhang, Qunye
Jiang, Fan
Source
Bioinorganic Chemistry and Applications
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-06-11
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Arsenic trioxide exhibits therapeutic effects on certain blood malignancies, at least partly by modulating cell differentiation.
Previous in vitro studies in human hematopoietic progenitor cells have suggested that arsenic may inhibit erythroid differentiation.
However, these effects were all observed in the presence of arsenic compounds, while the concomitant cytostatic and cytotoxic actions of arsenic might mask a prodifferentiating activity.
To eliminate the potential impacts of the cytostatic and cytotoxic actions of arsenic, we adopted a novel protocol by pretreating human bone marrow CD34+ cells with a low, noncytotoxic concentration of arsenic trioxide, followed by assaying the colony forming activities in the absence of the arsenic compound.
Bone marrow specimens were obtained from chronic myeloid leukemia patients who achieved complete cytogenetic remission.
CD34+ cells were isolated by magnetic-activated cell sorting.
We discovered that arsenic trioxide enhanced the erythroid colony forming activity, which was accompanied by a decrease in the granulomonocytic differentiation function.
Moreover, in erythroleukemic K562 cells, we showed that arsenic trioxide inhibited erythrocyte maturation, suggesting that arsenic might have biphasic effects on erythropoiesis.
In conclusion, our data provided the first evidence showing that arsenic trioxide could prime human hematopoietic progenitor cells for enhanced erythroid differentiation.
American Psychological Association (APA)
Zhang, Yuanyuan& Wang, Shasha& Chen, Chunyan& Wu, Xiao& Zhang, Qunye& Jiang, Fan. 2015. Arsenic Primes Human Bone Marrow CD34+ Cells for Erythroid Differentiation. Bioinorganic Chemistry and Applications،Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1054054
Modern Language Association (MLA)
Zhang, Yuanyuan…[et al.]. Arsenic Primes Human Bone Marrow CD34+ Cells for Erythroid Differentiation. Bioinorganic Chemistry and Applications No. 2015 (2015), pp.1-6.
https://search.emarefa.net/detail/BIM-1054054
American Medical Association (AMA)
Zhang, Yuanyuan& Wang, Shasha& Chen, Chunyan& Wu, Xiao& Zhang, Qunye& Jiang, Fan. Arsenic Primes Human Bone Marrow CD34+ Cells for Erythroid Differentiation. Bioinorganic Chemistry and Applications. 2015. Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1054054
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1054054