Protecting Intestinal Epithelial Cell Number 6 against Fission Neutron Irradiation through NF-κB Signaling Pathway
Joint Authors
Li, Jin-Feng
Chang, Gong-Min
Gao, Ya-Bing
Wang, Shui-Ming
Xu, Xin-Ping
Zhao, Li
Zhang, Jing
Peng, Rui-Yun
Wang, Yunliang
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-03-19
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The purpose of this paper is to explore the change of NF-κB signaling pathway in intestinal epithelial cell induced by fission neutron irradiation and the influence of the PI3K/Akt pathway inhibitor LY294002.
Three groups of IEC-6 cell lines were given: control group, neutron irradiation of 4Gy group, and neutron irradiation of 4Gy with LY294002 treatment group.
Except the control group, the other groups were irradiated by neutron of 4Gy.
LY294002 was given before 24 hours of neutron irradiation.
At 6 h and 24 h after neutron irradiation, the morphologic changes, proliferation ability, apoptosis, and necrosis rates of the IEC-6 cell lines were assayed and the changes of NF-κB and PI3K/Akt pathway were detected.
At 6 h and 24 h after neutron irradiation of 4Gy, the proliferation ability of the IEC-6 cells decreased and lots of apoptotic and necrotic cells were found.
The injuries in LY294002 treatment and neutron irradiation group were more serious than those in control and neutron irradiation groups.
The results suggest that IEC-6 cells were obviously damaged and induced serious apoptosis and necrosis by neutron irradiation of 4Gy; the NF-κB signaling pathway in IEC-6 was activated by neutron irradiation which could protect IEC-6 against injury by neutron irradiation; LY294002 could inhibit the activity of IEC-6 cells.
American Psychological Association (APA)
Chang, Gong-Min& Gao, Ya-Bing& Wang, Shui-Ming& Xu, Xin-Ping& Zhao, Li& Zhang, Jing…[et al.]. 2015. Protecting Intestinal Epithelial Cell Number 6 against Fission Neutron Irradiation through NF-κB Signaling Pathway. BioMed Research International،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1054265
Modern Language Association (MLA)
Chang, Gong-Min…[et al.]. Protecting Intestinal Epithelial Cell Number 6 against Fission Neutron Irradiation through NF-κB Signaling Pathway. BioMed Research International No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1054265
American Medical Association (AMA)
Chang, Gong-Min& Gao, Ya-Bing& Wang, Shui-Ming& Xu, Xin-Ping& Zhao, Li& Zhang, Jing…[et al.]. Protecting Intestinal Epithelial Cell Number 6 against Fission Neutron Irradiation through NF-κB Signaling Pathway. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1054265
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1054265