Constructing a Genome-Wide LD Map of Wild A. gambiae Using Next-Generation Sequencing
Joint Authors
Wang, Xiaohong
Afrane, Yaw A.
Yan, Guiyun
Li, Jun
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-09-03
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Anopheles gambiae is the major malaria vector in Africa.
Examining the molecular basis of A.
gambiae traits requires knowledge of both genetic variation and genome-wide linkage disequilibrium (LD) map of wild A.
gambiae populations from malaria-endemic areas.
We sequenced the genomes of nine wild A.
gambiae mosquitoes individually using next-generation sequencing technologies and detected 2,219,815 common single nucleotide polymorphisms (SNPs), 88% of which are novel.
SNPs are not evenly distributed across A.
gambiae chromosomes.
The low SNP-frequency regions overlay heterochromatin and chromosome inversion domains, consistent with the lower recombinant rates at these regions.
Nearly one million SNPs that were genotyped correctly in all individual mosquitoes with 99.6% confidence were extracted from these high-throughput sequencing data.
Based on these SNP genotypes, we constructed a genome-wide LD map for wild A.
gambiae from malaria-endemic areas in Kenya and made it available through a public Website.
The average size of LD blocks is less than 40 bp, and several large LD blocks were also discovered clustered around the para gene, which is consistent with the effect of insecticide selective sweeps.
The SNPs and the LD map will be valuable resources for scientific communities to dissect the A.
gambiae genome.
American Psychological Association (APA)
Wang, Xiaohong& Afrane, Yaw A.& Yan, Guiyun& Li, Jun. 2015. Constructing a Genome-Wide LD Map of Wild A. gambiae Using Next-Generation Sequencing. BioMed Research International،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1054703
Modern Language Association (MLA)
Wang, Xiaohong…[et al.]. Constructing a Genome-Wide LD Map of Wild A. gambiae Using Next-Generation Sequencing. BioMed Research International No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1054703
American Medical Association (AMA)
Wang, Xiaohong& Afrane, Yaw A.& Yan, Guiyun& Li, Jun. Constructing a Genome-Wide LD Map of Wild A. gambiae Using Next-Generation Sequencing. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1054703
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1054703