Effects of Mitochondrial Uncoupling Protein 2 Inhibition by Genipin in Human Cumulus Cells
Joint Authors
Ge, Hongshan
Zhang, Fan
Shan, Dan
Chen, Hua
Wang, Xiaona
Ling, Chao
Xi, HaiTao
Huang, Jianying
Zhu, ChunFang
Lv, Jeiqiang
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-08-19
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
UCP2 plays a physiological role by regulating mitochondrial biogenesis, maintaining energy balance, ROS elimination, and regulating cellular autophagy in numerous tissues.
But the exact roles of UCP2 in cumulus cells are still not clear.
Genipin, a special UCP2 inhibitor, was added into the cultural medium to explore the roles of UCP2 in human cumulus cells.
There were no significant differences in ATP and mitochondrial membrane potential levels in cumulus cells from UCP2 inhibiting groups as compared with the control.
The levels of ROS and Mn-SOD were markedly elevated after UCP2 inhibited Genipin.
However, the ratio of reduced GSH to GSSG significantly declined after treatment with Genipin.
UCP2 inhibition by Genipin also resulted in obvious increase in the active caspase-3, which accompanied the decline of caspase-3 mRNA.
The level of progesterone in culture medium declined obviously after Genipin treatment.
But there was no significant difference in estradiol concentrations.
This study indicated that UCP2 is expressed in human cumulus cells and plays important roles on mediate ROS production, apoptotic process, and steroidogenesis, suggesting UCP2 may be involved in regulation of follicle development and oocyte maturation and quality.
American Psychological Association (APA)
Ge, Hongshan& Zhang, Fan& Shan, Dan& Chen, Hua& Wang, Xiaona& Ling, Chao…[et al.]. 2015. Effects of Mitochondrial Uncoupling Protein 2 Inhibition by Genipin in Human Cumulus Cells. BioMed Research International،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1055067
Modern Language Association (MLA)
Ge, Hongshan…[et al.]. Effects of Mitochondrial Uncoupling Protein 2 Inhibition by Genipin in Human Cumulus Cells. BioMed Research International No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1055067
American Medical Association (AMA)
Ge, Hongshan& Zhang, Fan& Shan, Dan& Chen, Hua& Wang, Xiaona& Ling, Chao…[et al.]. Effects of Mitochondrial Uncoupling Protein 2 Inhibition by Genipin in Human Cumulus Cells. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1055067
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055067