Tetrahydrocannabinol Induces Brain Mitochondrial Respiratory Chain Dysfunction and Increases Oxidative Stress: A Potential Mechanism Involved in Cannabis-Related Stroke
Joint Authors
Schini-Kerth, Valérie B.
Wolff, Valérie
Zoll, Joffrey
Schlagowski, Anna-Isabel
Rouyer, Olivier
Singh, François
Marescaux, Christian
Raul, Jean-Sébastien
Geny, Bernard
Charles, Anne-Laure
Auger, Cyril
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-01-14
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Cannabis has potential therapeutic use but tetrahydrocannabinol (THC), its main psychoactive component, appears as a risk factor for ischemic stroke in young adults.
We therefore evaluate the effects of THC on brain mitochondrial function and oxidative stress, key factors involved in stroke.
Maximal oxidative capacities Vmax (complexes I, III, and IV activities), Vsucc (complexes II, III, and IV activities), Vtmpd (complex IV activity), together with mitochondrial coupling (Vmax/V0), were determined in control conditions and after exposure to THC in isolated mitochondria extracted from rat brain, using differential centrifugations.
Oxidative stress was also assessed through hydrogen peroxide (H2O2) production, measured with Amplex Red.
THC significantly decreased Vmax (−71%; P<0.0001), Vsucc (−65%; P<0.0001), and Vtmpd (−3.5%; P<0.001).
Mitochondrial coupling (Vmax/V0) was also significantly decreased after THC exposure (1.8±0.2 versus 6.3±0.7; P<0.001).
Furthermore, THC significantly enhanced H2O2 production by cerebral mitochondria (+171%; P<0.05) and mitochondrial free radical leak was increased from 0.01±0.01 to 0.10±0.01% (P<0.001).
Thus, THC increases oxidative stress and induces cerebral mitochondrial dysfunction.
This mechanism may be involved in young cannabis users who develop ischemic stroke since THC might increase patient’s vulnerability to stroke.
American Psychological Association (APA)
Wolff, Valérie& Schlagowski, Anna-Isabel& Rouyer, Olivier& Charles, Anne-Laure& Singh, François& Auger, Cyril…[et al.]. 2015. Tetrahydrocannabinol Induces Brain Mitochondrial Respiratory Chain Dysfunction and Increases Oxidative Stress: A Potential Mechanism Involved in Cannabis-Related Stroke. BioMed Research International،Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1055069
Modern Language Association (MLA)
Wolff, Valérie…[et al.]. Tetrahydrocannabinol Induces Brain Mitochondrial Respiratory Chain Dysfunction and Increases Oxidative Stress: A Potential Mechanism Involved in Cannabis-Related Stroke. BioMed Research International No. 2015 (2015), pp.1-7.
https://search.emarefa.net/detail/BIM-1055069
American Medical Association (AMA)
Wolff, Valérie& Schlagowski, Anna-Isabel& Rouyer, Olivier& Charles, Anne-Laure& Singh, François& Auger, Cyril…[et al.]. Tetrahydrocannabinol Induces Brain Mitochondrial Respiratory Chain Dysfunction and Increases Oxidative Stress: A Potential Mechanism Involved in Cannabis-Related Stroke. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1055069
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055069