Stem Cell Therapy for Corneal Epithelium Regeneration following Good Manufacturing and Clinical Procedures
Joint Authors
Fernández, Itziar
Ramírez, Beatriz E.
Sánchez, Ana
Herreras, José M.
García-Sancho, Javier
Nieto-Miguel, Teresa
Calonge, Margarita
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-19, 19 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-09-16
Country of Publication
Egypt
No. of Pages
19
Main Subjects
Abstract EN
Objective.
To evaluate outcomes of cultivated limbal epithelial transplantation (CLET) for management of ocular surface failure due to limbal stem cell deficiency (LSCD).
Design.
Prospective, noncomparative, interventional case series and extensive comparison with recent similar studies.
Participants.
Twenty eyes with LSCD underwent CLET (11 autologous; 9 allogeneic) and were followed up for 3 years.
Etiologies were divided into 3 prognostic categories: Group 1, chemical injuries (7 eyes); Group 2, immune-based inflammation (4 eyes); and Group 3, noninflammatory diseases (9 eyes).
Intervention.
Autologous and allogeneic limbal epithelial cells were cultivated on amniotic membranes and transplanted.
Evaluations were based on clinical parameters, survival analysis, and in vivo confocal microscopy (IVCM).
European Union Tissues/Cells Directive and good manufacturing procedures were followed.
Main Outcome Measures.
Improved clinical parameters, absence of epithelial defects, and improved central corneal epithelial phenotype.
Results.
Success rate was 80% at 1-2 years and 75% at 3 years.
Autografts and allografts had similar survival.
Success rate was significantly lower in prognostic Group 1 (42.9%) than in Groups 2-3 (100% each).
All clinical parameters improved substantially.
By IVCM, 80% of cases improved in epithelial status.
Conclusions.
CLET improved corneal epithelium quality, with subsequent improvement in symptoms, quality of life, and vision.
These results confirm that CLET is a valid therapy for ocular surface failure.
American Psychological Association (APA)
Ramírez, Beatriz E.& Sánchez, Ana& Herreras, José M.& Fernández, Itziar& García-Sancho, Javier& Nieto-Miguel, Teresa…[et al.]. 2015. Stem Cell Therapy for Corneal Epithelium Regeneration following Good Manufacturing and Clinical Procedures. BioMed Research International،Vol. 2015, no. 2015, pp.1-19.
https://search.emarefa.net/detail/BIM-1055370
Modern Language Association (MLA)
Ramírez, Beatriz E.…[et al.]. Stem Cell Therapy for Corneal Epithelium Regeneration following Good Manufacturing and Clinical Procedures. BioMed Research International No. 2015 (2015), pp.1-19.
https://search.emarefa.net/detail/BIM-1055370
American Medical Association (AMA)
Ramírez, Beatriz E.& Sánchez, Ana& Herreras, José M.& Fernández, Itziar& García-Sancho, Javier& Nieto-Miguel, Teresa…[et al.]. Stem Cell Therapy for Corneal Epithelium Regeneration following Good Manufacturing and Clinical Procedures. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-19.
https://search.emarefa.net/detail/BIM-1055370
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055370