A Novel Microbisporicin Producer Identified by Early Dereplication during Lantibiotic Screening
Joint Authors
Carrano, Lucia
Abbondi, Monica
Turconi, Paola
Candiani, Gianpaolo
Marinelli, Flavia
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-08-04
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
With the increasing need of effective antibiotics against multi-drug resistant pathogens, lantibiotics are an attractive option of a new class of molecules.
They are ribosomally synthetized and posttranslationally modified peptides possessing potent antimicrobial activity against aerobic and anaerobic Gram-positive pathogens, including those increasingly resistant to β-lactams and glycopeptides.
Some of them (actagardine, mersacidin, planosporicin, and microbisporicin) inhibit cell wall biosynthesis in pathogens and their effect is not antagonized by vancomycin.
Hereby, we apply an efficient strategy for lantibiotic screening to 240 members of a newly described genus of filamentous actinomycetes, named Actinoallomurus, that is considered a yet-poorly-exploited promising source for novel bioactive metabolites.
By combining antimicrobial differential assay against Staphylococcus aureus and its L-form (also in the presence of a β-lactamase cocktail or Ac-Lys-D-alanyl-D-alanine tripeptide), with LC-UV-MS dereplication coupled with bioautography, a novel producer of the potent microbisporicin complex was rapidly identified.
Under the commercial name of NAI-107, it is currently in late preclinical phase for the treatment of multi-drug resistant Gram-positive pathogens.
To our knowledge, this is the first report on a lantibiotic produced by an Actinoallomurus sp.
and on a microbisporicin producer not belonging to the Microbispora genus.
American Psychological Association (APA)
Carrano, Lucia& Abbondi, Monica& Turconi, Paola& Candiani, Gianpaolo& Marinelli, Flavia. 2015. A Novel Microbisporicin Producer Identified by Early Dereplication during Lantibiotic Screening. BioMed Research International،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1055421
Modern Language Association (MLA)
Carrano, Lucia…[et al.]. A Novel Microbisporicin Producer Identified by Early Dereplication during Lantibiotic Screening. BioMed Research International No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1055421
American Medical Association (AMA)
Carrano, Lucia& Abbondi, Monica& Turconi, Paola& Candiani, Gianpaolo& Marinelli, Flavia. A Novel Microbisporicin Producer Identified by Early Dereplication during Lantibiotic Screening. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1055421
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055421