Dissociation of Calcium Transients and Force Development following a Change in Stimulation Frequency in Isolated Rabbit Myocardium
Joint Authors
Janssen, Paul M. L.
Davis, Jonathan P.
Varian, Kenneth D.
Haizlip, Kaylan M.
Milani-Nejad, Nima
Brunello, Lucia
Slabaugh, Jessica L.
Walton, Shane D.
Gyorke, Sandor
Biesiadecki, Brandon J.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-04-15
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
As the heart transitions from one exercise intensity to another, changes in cardiac output occur, which are modulated by alterations in force development and calcium handling.
Although the steady-state force-calcium relationship at various heart rates is well investigated, regulation of these processes during transitions in heart rate is poorly understood.
In isolated right ventricular muscle preparations from the rabbit, we investigated the beat-to-beat alterations in force and calcium during the transition from one stimulation frequency to another, using contractile assessments and confocal microscopy.
We show that a change in steady-state conditions occurs in multiple phases: a rapid phase, which is characterized by a fast change in force production mirrored by a change in calcium transient amplitude, and a slow phase, which follows the rapid phase and occurs as the muscle proceeds to stabilize at the new frequency.
This second/late phase is characterized by a quantitative dissociation between the calcium transient amplitude and developed force.
Twitch timing kinetics, such as time to peak tension and 50% relaxation rate, reached steady-state well before force development and calcium transient amplitude.
The dynamic relationship between force and calcium upon a switch in stimulation frequency unveils the dynamic involvement of myofilament-based properties in frequency-dependent activation.
American Psychological Association (APA)
Haizlip, Kaylan M.& Milani-Nejad, Nima& Brunello, Lucia& Varian, Kenneth D.& Slabaugh, Jessica L.& Walton, Shane D.…[et al.]. 2015. Dissociation of Calcium Transients and Force Development following a Change in Stimulation Frequency in Isolated Rabbit Myocardium. BioMed Research International،Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1055594
Modern Language Association (MLA)
Haizlip, Kaylan M.…[et al.]. Dissociation of Calcium Transients and Force Development following a Change in Stimulation Frequency in Isolated Rabbit Myocardium. BioMed Research International No. 2015 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1055594
American Medical Association (AMA)
Haizlip, Kaylan M.& Milani-Nejad, Nima& Brunello, Lucia& Varian, Kenneth D.& Slabaugh, Jessica L.& Walton, Shane D.…[et al.]. Dissociation of Calcium Transients and Force Development following a Change in Stimulation Frequency in Isolated Rabbit Myocardium. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-12.
https://search.emarefa.net/detail/BIM-1055594
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055594