Cholinergic Synaptic Transmissions Were Altered after Single Sevoflurane Exposure in Drosophila Pupa
Joint Authors
Chen, Rongfa
Zhang, Tao
Kuang, Liting
Chen, Zhen
Ran, Dongzhi
Niu, Yang
Xu, Kangqing
Gu, Huaiyu
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-02-01
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Purpose.
Sevoflurane, one of the most used general anesthetics, is widely used in clinical practice all over the world.
Previous studies indicated that sevoflurane could induce neuron apoptosis and neural deficit causing query in the safety of anesthesia using sevoflurane.
The present study was designed to investigate the effects of sevoflurane on electrophysiology in Drosophila pupa whose excitatory neurotransmitter is acetylcholine early after sevoflurane exposure using whole brain recording technique.
Methods.
Wide types of Drosophila (canton-s flies) were allocated to control and sevoflurane groups randomly.
Sevoflurane groups (1% sevoflurane; 2% sevoflurane; 3% sevoflurane) were exposed to sevoflurane and the exposure lasted 5 hours, respectively.
All flies were subjected to electrophysiology experiment using patch clamp 24 hours after exposure.
Results.
The results showed that, 24 hours after sevoflurane exposure, frequency but not the amplitude of miniature excitatory postsynaptic currents (mEPSCs) was significantly reduced P<0.05.
Furthermore, we explored the underlying mechanism and found that calcium currents density, which partially regulated the frequency of mEPSCs, was significantly reduced after sevoflurane exposure P<0.05.
Conclusions.
All these suggested that sevoflurane could alter the mEPSCs that are related to synaptic plasticity partially through modulating calcium channel early after sevoflurane exposure.
American Psychological Association (APA)
Chen, Rongfa& Zhang, Tao& Kuang, Liting& Chen, Zhen& Ran, Dongzhi& Niu, Yang…[et al.]. 2015. Cholinergic Synaptic Transmissions Were Altered after Single Sevoflurane Exposure in Drosophila Pupa. BioMed Research International،Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1055659
Modern Language Association (MLA)
Chen, Rongfa…[et al.]. Cholinergic Synaptic Transmissions Were Altered after Single Sevoflurane Exposure in Drosophila Pupa. BioMed Research International No. 2015 (2015), pp.1-7.
https://search.emarefa.net/detail/BIM-1055659
American Medical Association (AMA)
Chen, Rongfa& Zhang, Tao& Kuang, Liting& Chen, Zhen& Ran, Dongzhi& Niu, Yang…[et al.]. Cholinergic Synaptic Transmissions Were Altered after Single Sevoflurane Exposure in Drosophila Pupa. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1055659
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055659