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Integrative Analysis of CRISPRCas9 Target Sites in the Human HBB Gene
Joint Authors
Luo, Yumei
Zhu, Detu
Pan, Shen Quan
Zhang, Zhizhuo
Chen, Yaoyong
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-03-31
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Recently, the clustered regularly interspaced short palindromic repeats (CRISPR) system has emerged as a powerful customizable artificial nuclease to facilitate precise genetic correction for tissue regeneration and isogenic disease modeling.
However, previous studies reported substantial off-target activities of CRISPR system in human cells, and the enormous putative off-target sites are labor-intensive to be validated experimentally, thus motivating bioinformatics methods for rational design of CRISPR system and prediction of its potential off-target effects.
Here, we describe an integrative analytical process to identify specific CRISPR target sites in the human β-globin gene (HBB) and predict their off-target effects.
Our method includes off-target analysis in both coding and noncoding regions, which was neglected by previous studies.
It was found that the CRISPR target sites in the introns have fewer off-target sites in the coding regions than those in the exons.
Remarkably, target sites containing certain transcriptional factor motif have enriched binding sites of relevant transcriptional factor in their off-target sets.
We also found that the intron sites have fewer SNPs, which leads to less variation of CRISPR efficiency in different individuals during clinical applications.
Our studies provide a standard analytical procedure to select specific CRISPR targets for genetic correction.
American Psychological Association (APA)
Luo, Yumei& Zhu, Detu& Zhang, Zhizhuo& Chen, Yaoyong& Pan, Shen Quan. 2015. Integrative Analysis of CRISPRCas9 Target Sites in the Human HBB Gene. BioMed Research International،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1055777
Modern Language Association (MLA)
Luo, Yumei…[et al.]. Integrative Analysis of CRISPRCas9 Target Sites in the Human HBB Gene. BioMed Research International No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1055777
American Medical Association (AMA)
Luo, Yumei& Zhu, Detu& Zhang, Zhizhuo& Chen, Yaoyong& Pan, Shen Quan. Integrative Analysis of CRISPRCas9 Target Sites in the Human HBB Gene. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1055777
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1055777